OVCAR-3细胞对顺铂和热疗的反应：热疗真的重要吗？
Response of OVCAR-3 Cells to Cisplatin and Hyperthermia: Does Hyperthermia Really Matter?
作者信息
Sukovas Arturas, Cesna Vaidotas, Jasukaitiene Aldona, Barauskas Giedrius, Nadisauskiene Ruta Jolanta, Dambrauskas Zilvinas, Paskauskas Saulius, Gulbinas Antanas
机构信息
Department of Obstetrics and Gynecology, Lithuanian University of Health Sciences, Kaunas, Lithuania
Department of Surgery, Lithuanian University of Health Sciences, Kaunas, Lithuania.
出版信息
Anticancer Res. 2017 Sep;37(9):5011-5018. doi: 10.21873/anticanres.11915.
BACKGROUND
Hyperthermic intraperitoneal chemotherapy (HIPEC) is proposed as a promising treatment method, but fundamental information about the contribution of hyperthermia to intraperitoneal chemotherapy is lacking. The purpose of this study was to investigate the cytotoxic effect of hyperthermia and cisplatin on OVCAR-3 cells in vitro.
MATERIALS AND METHODS
Imitating the typical clinical conditions of HIPEC, OVCAR-3 cells were exposed to hyperthermia and cisplatin for 1 h. MTT viability test, flow cytometric analysis, and real-time cell and isobologram analysis were performed.
RESULTS
Hyperthermia up to 42°C did not significantly increase the effect of cisplatin regarding the viability and apoptosis of OVCAR-3 cells. Moreover, an antagonistic effect of hyperthermia and cisplatin was revealed.
CONCLUSION
Our investigation of OVCAR-3 cells critically disputes the benefit of hyperthermia in ovarian cancer treatment. Further in vitro and in vivo research is essential for better understanding of the mechanisms of action of hyperthermia and its role in the treatment of epithelial ovarian cancer.
背景
腹腔热灌注化疗（HIPEC）被认为是一种很有前景的治疗方法，但关于热疗对腹腔化疗作用的基础信息尚缺乏。本研究的目的是在体外研究热疗和顺铂对OVCAR-3细胞的细胞毒性作用。
材料与方法
模拟HIPEC的典型临床条件，将OVCAR-3细胞暴露于热疗和顺铂中1小时。进行MTT活力测试、流式细胞术分析以及实时细胞和等效线图分析。
结果
高达42°C的热疗在OVCAR-3细胞的活力和凋亡方面未显著增强顺铂的作用。此外，还揭示了热疗和顺铂之间的拮抗作用。
结论
我们对OVCAR-3细胞的研究严重质疑了热疗在卵巢癌治疗中的益处。进一步的体外和体内研究对于更好地理解热疗的作用机制及其在上皮性卵巢癌治疗中的作用至关重要。
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