Schott Sonia, Ambrosini Arnaud, Barbaste Audrey, Benassayag Corinne, Gracia Mélanie, Proag Amsha, Rayer Mégane, Monier Bruno, Suzanne Magali
LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS/UPS, 118 route de Narbonne, 31062 Toulouse, France.
LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS/UPS, 118 route de Narbonne, 31062 Toulouse, France
Development. 2017 Oct 15;144(20):3840-3846. doi: 10.1242/dev.149807. Epub 2017 Sep 4.
Far from being passive, apoptotic cells influence their environment. For example, they promote tissue folding, myoblast fusion and modulate tumor growth. Understanding the role of apoptotic cells necessitates their efficient tracking within living tissues, a task that is currently challenging. In order to easily spot apoptotic cells in developing tissues, we generated a series of fly lines expressing different fluorescent sensors of caspase activity. We show that three of these reporters (GFP-, Cerulean- and Venus-derived molecules) are detected specifically in apoptotic cells and throughout the whole process of programmed cell death. These reporters allow the specific visualization of apoptotic cells directly within living tissues, without any post-acquisition processing. They overcome the limitations of other apoptosis detection methods developed so far and, notably, they can be combined with any kind of fluorophore.
凋亡细胞远非被动，它们会影响周围环境。例如，它们促进组织折叠、成肌细胞融合并调节肿瘤生长。要了解凋亡细胞的作用，就需要在活组织中对其进行有效追踪，而目前这仍是一项具有挑战性的任务。为了在发育中的组织中轻松识别凋亡细胞，我们构建了一系列表达不同半胱天冬酶活性荧光传感器的果蝇品系。我们发现其中三种报告基因（源自绿色荧光蛋白、天蓝荧光蛋白和黄色荧光蛋白的分子）在凋亡细胞中以及整个程序性细胞死亡过程中均能被特异性检测到。这些报告基因能够直接在活组织中对凋亡细胞进行特异性可视化，无需任何采集后处理。它们克服了迄今为止开发的其他凋亡检测方法的局限性，尤其可以与任何种类的荧光团结合使用。
