开花植物中AOX蛋白分类的分步方案
A Step-by-Step Protocol for Classifying AOX Proteins in Flowering Plants.
作者信息
Costa José Hélio, Dos Santos Clesivan Pereira, da Cruz Saraiva Kátia Daniella, Arnholdt-Schmitt Birgit
机构信息
Functional Genomics and Bioinformatics, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Functional Cell Reprogramming and Organism Plasticity (FunCrop), EU Marie Curie Chair, ICAAM, Universidade de Évora, Apartado 94, 7002-554, Évora, Portugal.
出版信息
Methods Mol Biol. 2017;1670:225-234. doi: 10.1007/978-1-4939-7292-0_19.
The potential of alternative oxidase (AOX) genes to develop functional markers for plant breeding programs has been emphasized. In this sense, it is essential to have a reliable classification system, which could aid in the selection of candidate AOX genes from different species. In the case of angiosperms AOX, a robust classification system is required because this enzyme is encoded by variable gene numbers (1-6 genes) with variable AOX subfamilies and subtypes. Thus, in this protocol, we present a detailed guideline to application of a classification scheme of AOX based on specific amino acids and phylogeny. We believe that this classification protocol provides an easier and practical way of classifying new angiosperm AOX genes besides that it can help to standardize AOX gene names used in AOX research community.
替代氧化酶（AOX）基因在植物育种计划中开发功能标记的潜力已得到强调。从这个意义上说，拥有一个可靠的分类系统至关重要，这有助于从不同物种中选择候选AOX基因。对于被子植物AOX而言，需要一个强大的分类系统，因为这种酶由数量可变的基因（1 - 6个基因）编码，具有可变的AOX亚家族和亚型。因此，在本方案中，我们提出了一个基于特定氨基酸和系统发育的AOX分类方案应用的详细指南。我们相信，这个分类方案除了有助于规范AOX研究领域中使用的AOX基因名称外，还为新的被子植物AOX基因分类提供了一种更简便实用的方法。
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