Sunil Bobba, Raghavendra Agepati S
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India.
Methods Mol Biol. 2017;1670:253-265. doi: 10.1007/978-1-4939-7292-0_22.
The electron partitioning between COX and AOX pathways of mitochondria and their coordination is necessary to meet the energy demands as well as to maintain optimized redox status in plants under varying environmental conditions. The relative contribution of these two pathways to total respiration is an important measure during a given stress condition. We describe in detail the procedure that allows the measurement of the parameters of COX and AOX pathway of respiration in mesophyll protoplasts using Clark-type O electrode. This chapter also lists the steps for rapid isolation procedure for mesophyll protoplasts from pea leaves. The advantages and limitations of the use of metabolic inhibitors and the protoplasts for measuring the respiration are also briefly discussed.
线粒体中细胞色素c氧化酶（COX）和交替氧化酶（AOX）途径之间的电子分配及其协调，对于满足植物在不同环境条件下的能量需求以及维持优化的氧化还原状态是必要的。在特定胁迫条件下，这两条途径对总呼吸的相对贡献是一项重要指标。我们详细描述了使用克拉克型氧电极测量叶肉原生质体中COX和AOX呼吸途径参数的方法。本章还列出了从豌豆叶片中快速分离叶肉原生质体的步骤。还简要讨论了使用代谢抑制剂和原生质体测量呼吸作用的优点和局限性。
