Rodríguez-López Pedro, Puga Carmen H, Orgaz Belén, Cabo Marta L
a Department of Microbiology and Technology of Marine Products , Instituto de Investigaciones Marinas (IIM-CSIC) , Pontevedra , Spain.
b Department of Nutrition, Food Science and Technology, Faculty of Veterinary , University Complutense of Madrid (UCM) , Madrid , Spain.
Biofouling. 2017 Sep;33(8):690-702. doi: 10.1080/08927014.2017.1356290.
This work presents the assessment of the effectivity of a pronase (PRN)-benzalkonium chloride (BAC) sequential treatment in removing Listeria monocytogenes-Escherichia coli dual-species biofilms grown on stainless steel (SS) using fluorescence microscopy and plate count assays. The effects of PRN-BAC on the occupied area (OA) by undamaged cells in 168 h dual-species samples were determined using a first-order factorial design. Empirical equations significantly (r = 0.927) described a negative individual effect of BAC and a negative interactive effect of PRN-BAC achieving OA reductions up to 46%. After treatment, high numbers of remaining attached and released viable and cultivable E. coli cells were detected in PRN-BAC combinations when low BAC concentrations were used. Therefore, at appropriate BAC doses, in addition to biofilm removal, sequential application of PRN and BAC represents an appealing strategy for pathogen control on SS surfaces while hindering the dispersion of live cells into the environment.
本研究通过荧光显微镜和平板计数法，评估了链霉蛋白酶（PRN）-苯扎氯铵（BAC）序贯处理对不锈钢（SS）表面生长的单核细胞增生李斯特菌-大肠杆菌双物种生物膜的去除效果。采用一阶析因设计确定PRN-BAC对168小时双物种样本中未受损细胞所占面积（OA）的影响。经验方程显著（r = 0.927）描述了BAC的负个体效应以及PRN-BAC的负交互效应，OA减少高达46%。处理后，当使用低BAC浓度时，在PRN-BAC组合中检测到大量残留附着和释放的存活且可培养的大肠杆菌细胞。因此，在适当的BAC剂量下，除了去除生物膜外，PRN和BAC的序贯应用是一种有吸引力的控制SS表面病原体的策略，同时可防止活细胞扩散到环境中。
