Rodríguez-López Pedro, Rodríguez-Herrera Juan José, López Cabo Marta
Laboratory of Microbiology and Technology of Marine Products (MICROTEC), Instituto de Investigacións Mariñas (IIM-CSIC), Vigo, Spain.
Front Microbiol. 2022 Jun 9;13:917964. doi: 10.3389/fmicb.2022.917964. eCollection 2022.
is considered a foodborne pathogen of serious concern capable of forming multispecies biofilms with other bacterial species, such as spp., adhered onto stainless steel (SS) surfaces. In an attempt to link the biofilms' morphology and resistance to biocides, dual-species biofilms of , in co-culture with either , , or , were assayed to ascertain their morphological characteristics and resistance toward benzalkonium chloride (BAC) and neutral electrolyzed water (NEW). Epifluorescence microscopy analysis revealed that each dual-species biofilm was distributed differently over the SS surface and that these differences were attributable to the presence of spp. Confocal laser scanning microscopy (CLSM) assays demonstrated that despite these differences in distribution, all biofilms had similar maximum thicknesses. Along with this, colocalization analyses showed a strong trend of to share location within the biofilm with all assayed whilst the latter distributed throughout the surface independently of the presence of , a fact that was especially evident in those biofilms in which cell clusters were present. Finally, a modified Gompertz equation was used to fit biofilms' BAC and NEW dose-response data. Outcomes demonstrated that was less susceptible to BAC when co-cultured with or , whereas susceptibility to NEW was reduced in all three dual-species biofilms, which can be attributable to both the mechanism of action of the biocide and the architectural features of each biofilm. Therefore, the results herein provided can be used to optimize already existing and develop novel target-specific sanitation treatments based on the mechanism of action of the biocide and the biofilms' species composition and structure.
被认为是一种备受关注的食源性病原体，能够与其他细菌物种形成多物种生物膜，例如附着在不锈钢（SS）表面的 spp.。为了将生物膜的形态与对杀菌剂的抗性联系起来，对 与 、 或 共培养的双物种生物膜进行了分析，以确定它们的形态特征以及对苯扎氯铵（BAC）和中性电解水（NEW）的抗性。落射荧光显微镜分析表明，每种双物种生物膜在SS表面的分布不同，这些差异归因于 spp.的存在。共聚焦激光扫描显微镜（CLSM）分析表明，尽管分布存在这些差异，但所有生物膜的最大厚度相似。与此同时，共定位分析显示，在所检测的所有 中， 与 在生物膜内共享位置的趋势很强，而后者独立于 的存在分布在整个表面，这一事实在存在细胞簇的生物膜中尤为明显。最后，使用修正的Gompertz方程拟合生物膜的BAC和NEW剂量反应数据。结果表明，与 或 共培养时， 对BAC的敏感性较低，而在所有三种双物种生物膜中对NEW的敏感性均降低，这可归因于杀菌剂的作用机制和每种生物膜的结构特征。因此，本文提供的结果可用于基于杀菌剂的作用机制以及生物膜的物种组成和结构，优化现有方法并开发新的针对特定靶点的卫生处理方法。
