Itai Shunsuke, Yamada Shinji, Kaneko Mika K, Harada Hiroyuki, Kato Yukinari
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Miyagi, Japan .
2 Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
Monoclon Antib Immunodiagn Immunother. 2017 Oct;36(5):220-223. doi: 10.1089/mab.2017.0030. Epub 2017 Sep 5.
Podocalyxin is a CD34-related type I transmembrane protein that is highly glycosylated with N-glycan, O-glycan, and keratan sulfate. Podocalyxin was originally found in the podocytes of rat kidney and is reportedly expressed in many types of tumors, including brain tumors, colorectal cancers, and breast cancers. Overexpression of podocalyxin is an independent predictor of progression, metastasis, and poor outcome. We recently immunized mice with recombinant human podocalyxin, which was produced using LN229 glioblastoma cells, and produced a novel antipodocalyxin monoclonal antibody (mAb), PcMab-47, which reacts with endogenous podocalyxin-expressing cancer cell lines and normal cell lines independent of glycosylation in Western blot, flow cytometry, and immunohistochemical analyses. In this study, we performed immunohistochemical analysis against oral cancers using PcMab-47. PcMab-47-stained oral squamous cell carcinoma cells in a cytoplasmic pattern and detected 26/38 (68.4%) of oral squamous cell carcinoma cells on tissue microarrays. These results indicate that PcMab-47 is useful in detecting podocalyxin of oral cancers for immunohistochemical analysis.
足细胞蛋白是一种与CD34相关的I型跨膜蛋白，高度糖基化，含有N-聚糖、O-聚糖和硫酸角质素。足细胞蛋白最初在大鼠肾脏的足细胞中发现，据报道在多种肿瘤中表达，包括脑肿瘤、结直肠癌和乳腺癌。足细胞蛋白的过表达是进展、转移和不良预后的独立预测指标。我们最近用重组人足细胞蛋白免疫小鼠，该重组人足细胞蛋白是用LN229胶质母细胞瘤细胞产生的，并产生了一种新型抗足细胞蛋白单克隆抗体（mAb），即PcMab-47，在蛋白质印迹、流式细胞术和免疫组织化学分析中，它能与内源性表达足细胞蛋白的癌细胞系和正常细胞系反应，且与糖基化无关。在本研究中，我们用PcMab-47对口腔癌进行了免疫组织化学分析。PcMab-47以细胞质模式对口腔鳞状细胞癌细胞进行染色，并在组织微阵列上检测到38个口腔鳞状细胞癌细胞中的26个（68.4%）。这些结果表明，PcMab-47可用于免疫组织化学分析检测口腔癌的足细胞蛋白。
