Yamada Shinji, Itai Shunsuke, Kaneko Mika K, Kato Yukinari
Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .
Monoclon Antib Immunodiagn Immunother. 2018 Apr;37(2):91-94. doi: 10.1089/mab.2018.0002. Epub 2018 Mar 14.
Lung cancer is one of the leading causes of cancer-related deaths in the world. Regardless of the advances in lung cancer treatments, the prognosis is still poor. Podocalyxin (PODXL) is a highly glycosylated type I transmembrane protein that is expressed in normal tissues, including the heart, pancreas, and breast. It is also found and used as a diagnostic marker in many cancers, such as renal, brain, breast, oral, and lung cancers. We previously developed specific and sensitive anti-PODXL monoclonal antibodies, PcMab-47 (mouse IgG, kappa) and its mouse IgG-type (47-mG), both of which were suitable for immunohistochemical analyses of oral cancers. In this study, we investigated the utility of PcMab-47 and 47-mG for the immunohistochemical analyses of lung cancers. PcMab-47 stained 51/70 (72.9%) cases of lung cancer, whereas 47-mG stained 59/70 (84.3%) cases, indicating that the latter antibody is more sensitive and is useful for detecting PODXL in lung cancers.
肺癌是全球癌症相关死亡的主要原因之一。尽管肺癌治疗取得了进展，但其预后仍然很差。足细胞毒素（PODXL）是一种高度糖基化的I型跨膜蛋白，在包括心脏、胰腺和乳腺在内的正常组织中表达。它也在许多癌症中被发现并用作诊断标志物，如肾癌、脑癌、乳腺癌、口腔癌和肺癌。我们之前开发了特异性和敏感性高的抗PODXL单克隆抗体PcMab-47（小鼠IgG，κ链）及其小鼠IgG型（47-mG），两者均适用于口腔癌的免疫组织化学分析。在本研究中，我们研究了PcMab-47和47-mG在肺癌免疫组织化学分析中的应用。PcMab-47对70例肺癌中的51例（72.9%）染色阳性，而47-mG对70例中的59例（84.3%）染色阳性，表明后一种抗体更敏感，可用于检测肺癌中的PODXL。
