a School of Biotechnology , Gautam Buddha University , Greater Noida , U.P. India.
Bioengineered. 2018 Jan 1;9(1):152-158. doi: 10.1080/21655979.2017.1373537. Epub 2017 Sep 21.
De novo synthesis of folates in plants is tightly regulated through feedback-regulation of certain pathway catalysts. Recently, we investigated the prospects of incessant production of folates in an evolutionary conjunction, through the overexpression of feedback targeted and evolutionarily conserved heterologous E.coli dihydroneopterin aldolase (EcDHNA) in tobacco. The enhanced production of folates in the transgenic lines was associated with differential allosteric regulatory cavities accessible at EcDHNA surface having critical amino-acid differences as Ile 64 (His_63), Val 70 (Phe_69), His 75 (Arg_78) and Arg 79 (Glu_72). These structural characteristics are indicative of evolutionary signatures of the catalytic feedback-regulation of folate manufacturing. We exploited the biotechnological potential of such allosterically diverged trans-DHNA for improved folate production in plants. Nonetheless, genetic manipulation of single enzymes modulating complex pathways such as folate biosynthesis is often inadequate to achieve desired phenotypes; therefore, multi-gene integration with explicit genic-combination for folate enrichment in plants has also been projected for future folate agri-biofortification schemes.
植物中叶酸的从头合成受到特定途径催化剂的反馈调节的严格控制。最近，我们通过在烟草中过量表达反馈靶向和进化上保守的异源大肠杆菌二氢喋呤醛缩酶（EcDHNA），研究了在进化结合中不断生产叶酸的可能性。在转基因系中叶酸的产量增加与 EcDHNA 表面可及的不同变构调节腔有关，这些调节腔具有关键的氨基酸差异，如 Ile64（His63）、Val70（Phe69）、His75（Arg78）和 Arg79（Glu72）。这些结构特征表明叶酸制造的催化反馈调节的进化特征。我们利用这种变构差异的反-DHNA 的生物技术潜力，提高植物中的叶酸产量。然而，遗传操纵单个调节复杂途径（如叶酸生物合成）的酶通常不足以实现所需的表型；因此，还计划在未来的叶酸农业生物强化计划中进行多基因整合，以明确的基因组合进行叶酸富集。
