Xu Lei, Jia Fei, Luo Changyao, Yu Qianqian, Dai Ruitong, Li Xingmin
College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
Food Chem. 2018 Jan 15;239:657-663. doi: 10.1016/j.foodchem.2017.06.128. Epub 2017 Jun 23.
Unfertilized chicken eggs within 24h of laying were chosen and stored at 25°C and 45% humidity for 0, 20, and 40days. The experimental group (EG) was the carbon dioxide-modified atmosphere packaging (CDMAP) group, whereas the control group (CG) contained eggs without special handling. Egg freshness indexes were measured. The proteome of the egg whites was determined by LC-MS/MS using isobaric tags for relative and absolute quantitation (iTRAQ). A total of 87 proteins were detected. The results indicated that CDMAP can control the change in protein abundance. Using a correlation analysis between the protein abundance and freshness indexes of the EG, Beta-hexosaminidase, Trypsin inhibitor ClTI-1 and Apolipoprotein D were determined to be potential predictors of egg freshness. In comparing the proteomes of the EG and CG, it was concluded that CDMAP could affect the proteins related to egg vitelline membranes, eggshell matrix and metabolic intensity to maintain egg freshness.
选取产蛋后24小时内的未受精鸡蛋，在25°C和45%湿度条件下储存0、20和40天。实验组（EG）为二氧化碳气调包装（CDMAP）组，而对照组（CG）包含未经过特殊处理的鸡蛋。测定鸡蛋新鲜度指标。蛋清的蛋白质组通过液相色谱-串联质谱（LC-MS/MS）使用等压标签相对和绝对定量（iTRAQ）进行测定。共检测到87种蛋白质。结果表明，CDMAP可以控制蛋白质丰度的变化。通过对EG组蛋白质丰度与新鲜度指标进行相关性分析，确定β-己糖胺酶、胰蛋白酶抑制剂ClTI-1和载脂蛋白D为鸡蛋新鲜度的潜在预测指标。比较EG组和CG组的蛋白质组后得出结论，CDMAP可影响与卵黄膜、蛋壳基质和代谢强度相关的蛋白质，以保持鸡蛋新鲜度。
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