D'Ambrosio Chiara, Arena Simona, Scaloni Andrea, Guerrier Luc, Boschetti Egisto, Mendieta Martha Elena, Citterio Attilio, Righetti Pier Giorgio
Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, Naples, Italy.
J Proteome Res. 2008 Aug;7(8):3461-74. doi: 10.1021/pr800193y. Epub 2008 Jun 21.
The use of two types of peptide ligand libraries (PLL), containing hexapeptides terminating either with a primary amine or modified with a terminal carboxyl group, allowed the discovery and identification of a large number of previously unreported egg white proteins. Whereas the most comprehensive list up to date ( Mann, K. , Proteomics 2007, 7, 3558- 3568 ) tabulated 78 unique gene products, our findings have almost doubled that value to 148 unique protein species. From the initial nontreated egg, it was possible to find 41 protein species; the difference (107 proteins) was generated as a result of the use of PLLs from which a similar number of species (112 and 109, respectively) was evidenced. Of those, 35 proteins were the specific catch of the amino-terminus PLL, while 33 were uniquely captured by the carboxy-terminus PLL. While a number of these low-abundance proteins might have a biological role in maintaining the integrity of the egg white and protecting the yolk, others might be derived from decaying epithelial cells lining the oviduct and/or represent remnants of products from the magnum and eggshell membrane components secreted by the isthmus, which might ultimately be incorporated, even if in trace amounts, into the egg white. The list of egg white components here reported is by far the most comprehensive at present and could serve as a starting point for isolation and functional characterization of proteins possibly having novel pharmaceutical and biomedical applications.
使用两种类型的肽配体文库（PLL），其中包含以伯胺结尾或用末端羧基修饰的六肽，这使得人们发现并鉴定出大量以前未报道的蛋清蛋白。虽然迄今为止最全面的列表（Mann, K., Proteomics 2007, 7, 3558 - 3568）列出了78种独特的基因产物，但我们的发现几乎使这个数值翻倍，达到了148种独特的蛋白质种类。从最初未经处理的鸡蛋中，能够找到41种蛋白质种类；差异（107种蛋白质）是由于使用PLL产生的，从中分别证实了相似数量的种类（分别为112种和109种）。其中，35种蛋白质是氨基末端PLL的特异性捕获物，而33种是羧基末端PLL独特捕获的。虽然这些低丰度蛋白质中的一些可能在维持蛋清的完整性和保护蛋黄方面具有生物学作用，但其他一些可能来自输卵管内衬的腐烂上皮细胞和/或代表来自峡部分泌的蛋白和蛋壳膜成分的产物残余，这些最终可能即使以微量形式也被纳入蛋清中。这里报道的蛋清成分列表是目前为止最全面的，并且可以作为分离和功能表征可能具有新型药物和生物医学应用的蛋白质的起点。
