INRA, UR83 Recherches Avicoles, Fonction et Régulation des Protéines de l'Œuf, F-37380 Nouzilly, France.
J Agric Food Chem. 2014 Mar 26;62(12):2531-40. doi: 10.1021/jf404512x. Epub 2014 Mar 18.
To better appreciate the dynamics of yolk proteins during embryonic development, we analyzed the protein quantitative changes occurring in the yolk plasma at the day of lay and after 12 days of incubation, by comparing unfertilized and fertilized chicken eggs. Of the 127 identified proteins, 69 showed relative abundance differences among conditions. Alpha-fetoprotein and two uncharacterized proteins (F1NHB8 and F1NMM2) were identified for the first time in the egg. After 12 days of incubation, five proteins (vitronectin, α-fetoprotein, similar to thrombin, apolipoprotein B, and apovitellenin-1) showed a major increase in relative abundance, whereas 15 proteins showed a significant decrease in the yolks of fertilized eggs. In unfertilized/table eggs, we observed an accumulation of proteins likely to originate from other egg compartments during incubation. This study provides basic knowledge on the utilization of egg yolk proteins by the embryo and gives some insight into how storage can affect egg quality.
为了更好地了解卵黄蛋白在胚胎发育过程中的动态变化，我们通过比较未受精和受精的鸡卵，分析了产蛋日和孵化 12 天后卵黄血浆中发生的蛋白质定量变化。在鉴定出的 127 种蛋白质中，有 69 种在不同条件下表现出相对丰度差异。甲胎蛋白和两种未鉴定的蛋白质（F1NHB8 和 F1NMM2）首次在鸡蛋中被发现。孵化 12 天后，5 种蛋白质（纤连蛋白、甲胎蛋白、类似凝血酶、载脂蛋白 B 和卵黄高磷蛋白-1）的相对丰度显著增加，而 15 种蛋白质在受精卵的卵黄中显著减少。在未受精/商品蛋中，我们观察到在孵化过程中，可能有来自其他卵室的蛋白质积累。本研究为胚胎对卵黄蛋白的利用提供了基础知识，并深入了解了储存如何影响鸡蛋质量。
