Chen Jiu-Yan, Cao Shu-Rui, Xi Cun-Xian, Chen Yu, Li Xian-Liang, Zhang Lei, Wang Guo-Min, Chen Ya-Ling, Chen Zhi-Qiong
College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.
The Inspection Technical Center of Chongqing Entry-Exit Inspection & Quarantine Bureau, Chongqing 400020, China.
Food Chem. 2018 Jan 15;239:911-919. doi: 10.1016/j.foodchem.2017.07.013. Epub 2017 Jul 8.
β-Cyclodextrin (β-CD) modified magnetic graphene oxide material (FeO@SiO/GO/β-CD), a novel magnetic sorbent for enrichment and purification of plant growth regulators (PGRs) in vegetables prior to gas chromatograph mass spectrometer (GC-MS) detection, was successfully fabricated. The novel magnetic sorbent geometrically fitted to selectively recognize and enrich PGR analytes especially containing aromatic structure. The selectivity experiment demonstrated the higher selectivity of FeO@SiO/GO/β-CD toward 5 PGR compounds than that of FeO@SiO/GO. Several parameters affecting extraction efficiency including the volume of extraction solvent, amount of adsorbent, extraction time, type and the volume of desorption solvent were optimized. Under the optimized condition, good linear relationships were obtained in the range of 1-100μg/kg with determination coefficients (R) from 0.9983 to 0.9996. The limits of detection (LODs) of 5 PGRs were from 0.04 to 0.28μg/kg. The proposed method had good potential for the analysis of trace level of 5 PGRs in real vegetable samples.
成功制备了β-环糊精（β-CD）修饰的磁性氧化石墨烯材料（FeO@SiO/GO/β-CD），这是一种新型磁性吸附剂，用于在气相色谱-质谱联用仪（GC-MS）检测之前对蔬菜中的植物生长调节剂（PGRs）进行富集和净化。这种新型磁性吸附剂在几何结构上适合选择性识别和富集特别是含有芳香结构的PGR分析物。选择性实验表明，FeO@SiO/GO/β-CD对5种PGR化合物的选择性高于FeO@SiO/GO。对影响萃取效率的几个参数进行了优化，包括萃取溶剂的体积、吸附剂的用量、萃取时间、解吸溶剂的类型和体积。在优化条件下，在1-100μg/kg范围内获得了良好的线性关系，测定系数（R）为0.9983至0.9996。5种PGR的检测限（LOD）为0.04至0.28μg/kg。该方法在实际蔬菜样品中痕量5种PGR的分析方面具有良好的潜力。
