Department of Biological Sciences, University of Limerick, Limerick, Ireland.
Food Res Int. 2017 Oct;100(Pt 1):416-422. doi: 10.1016/j.foodres.2017.07.037. Epub 2017 Jul 17.
Proteins derived from the macroalgal species Palmaria palmata have emerged as potential substrates for the generation of bioactive peptides. The aim of this study was to fractionate, identify and characterize antioxidant peptides from a P. palmata protein hydrolysate. The P. palmata protein hydrolysate generated with the food-grade proteolytic enzyme Corolase PP was sequentially fractionated using solid phase extraction and semi-preparative (SP) RP-HPLC. The most active SP-RP-HPLC peptide fraction (SP-RP-HPLC-30-F26) was analysed by ESI-MS/MS. Seventeen novel peptide sequences were identified in this fraction. Of the peptides selected for synthesis, Ser-Asp-Ile-Thr-Arg-Pro-Gly-Gly-Asn-Met, showed the highest oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) activity with values of 152.43±2.73 and 21.23±0.90nmolTE/μmol peptide, respectively. The results presented herein indicate that P. palmata derived peptides may have potential applications as health enhancing ingredients and as food preservatives due to their antioxidant activity.
从大型海藻掌状红皮藻中提取的蛋白质已成为生物活性肽的潜在底物。本研究旨在对掌状红皮藻蛋白水解物进行分离、鉴定和表征抗氧化肽。用食品级蛋白酶 Corolase PP 生成的掌状红皮藻蛋白水解物，采用固相萃取和半制备（SP）反相高效液相色谱（RP-HPLC）进行连续分级。最活跃的 SP-RP-HPLC 肽级分（SP-RP-HPLC-30-F26）通过 ESI-MS/MS 进行分析。在该级分中鉴定出 17 种新型肽序列。在所选择用于合成的肽中，丝氨酰-天冬氨酰-异亮氨酰-苏氨酰-精氨酰-脯氨酰-甘氨酰-甘氨酰-天冬酰胺-甲硫氨酸，表现出最高的氧自由基吸收能力（ORAC）和铁还原抗氧化能力（FRAP）活性，值分别为 152.43±2.73 和 21.23±0.90nmolTE/μmol 肽。本文的研究结果表明，掌状红皮藻衍生肽由于其抗氧化活性，可能具有作为增强健康成分和食品防腐剂的应用潜力。
