糖尿病对灌注大鼠肾脏在哇巴因和依他尼酸存在情况下利钠作用的影响。

Effect of diabetes on natriuresis in the presence of ouabain and ethacrynic acid in perfused rat kidney.

Bikhazi A B, Shukri R P

Comp Biochem Physiol A Comp Physiol. 1987;87(4):1063-6. doi: 10.1016/0300-9629(87)90040-5.

Abstract

The effect of diabetes on renal sodium retention was investigated. 2. The technique involved retrograde perfusion from the renal veins via the kidneys, and then through the renal arteries and dorsal aorta. 3. Sodium retention by diabetic rat kidney was 58% lower than that in the normal rats. 4. Ouabain (15 mM) in perfusate increased sodium retention by 30% in normal rat kidney as compared to a 54% increase in diabetic rat kidney. 5. Ethacrynic acid (1 mM) in perfusate resulted in a 42% reduction in sodium retention in the normal rat kidney as compared to a 43% decrease in the diabetic rat kidney. 6. Control of hyperglycemia in diabetic rats with insulin therapy resulted in sodium retention that is not significantly different from that of normal rats. 7. The results suggest that diabetes has no effect on the peritubular ouabain-sensitive Na--K-ATPase pump, or the luminal ethacrynic acid-sensitive Na-K counter transport pump. Furthermore, the data suggest a reversible effect of diabetes on sodium retention during insulin therapy.

摘要

研究了糖尿病对肾脏钠潴留的影响。2. 该技术包括从肾静脉经肾脏进行逆行灌注，然后通过肾动脉和背主动脉。3. 糖尿病大鼠肾脏的钠潴留比正常大鼠低58%。4. 灌注液中哇巴因（15 mM）使正常大鼠肾脏的钠潴留增加30%，而糖尿病大鼠肾脏增加54%。5. 灌注液中依他尼酸（1 mM）使正常大鼠肾脏的钠潴留减少42%，糖尿病大鼠肾脏减少43%。6. 用胰岛素治疗糖尿病大鼠的高血糖，其钠潴留与正常大鼠无显著差异。7. 结果表明，糖尿病对肾小管周围哇巴因敏感的钠钾ATP酶泵或管腔依他尼酸敏感的钠钾逆向转运泵没有影响。此外，数据表明糖尿病对胰岛素治疗期间的钠潴留具有可逆作用。