哇巴因和利尿剂对灌注大鼠肾脏钠、钾和氯潴留的作用。

Role of ouabain and diuretics on sodium, potassium and chloride retention in perfused rat kidney.

作者信息

Bikhazi A B, Zantut H, Maarawi N, Sayyid K

出版信息

Comp Biochem Physiol A Comp Physiol. 1984;77(3):507-11. doi: 10.1016/0300-9629(84)90219-6.

Abstract

A novel in situ kidney perfusion technique is described in Sprague-Dawley rats. The procedure involves retrograde perfusion from the renal veins via the kidneys, and then through the renal arteries and dorsal aorta. Ouabain (15 mM) in perfusate increased Na retention by 92%, decreased K retention by 53% and produced no effect on Cl retention. Ethacrynic acid (1 mM) in perfusate decreased Na retention by 52%, increased K retention by 105% and decreased Cl retention by 27%. Furosemide (1.5 mM) in perfusate decreased Na retention by 52%, increased K retention by 47% and decreased Cl retention by 56%. The Na-K-ATPase pump localized at the peritubular side of the proximal tubule cell is ouabain sensitive and Mg dependent. An Na-K pump responsible for Na influx and K effux exists at the luminal side of the proximal tubule cell and is ethacrynic acid and furosemide sensitive.

摘要

本文描述了一种在斯普拉格-道利大鼠中使用的新型原位肾脏灌注技术。该操作包括通过肾脏从肾静脉进行逆行灌注，然后通过肾动脉和背主动脉。灌注液中加入哇巴因（15 mM）使钠潴留增加92%，钾潴留减少53%，对氯潴留无影响。灌注液中加入依他尼酸（1 mM）使钠潴留减少52%，钾潴留增加105%，氯潴留减少27%。灌注液中加入呋塞米（1.5 mM）使钠潴留减少52%，钾潴留增加47%，氯潴留减少56%。位于近端小管细胞肾小管周围侧的钠钾ATP酶泵对哇巴因敏感且依赖镁。负责钠内流和钾外流的钠钾泵存在于近端小管细胞的管腔侧，对依他尼酸和呋塞米敏感。