Li Fang, Xu Limei, Yang Feng
State Key Laboratory Breeding Base of Marine Genetic Resources; Fujian Key Laboratory of Marine Genetic Resources; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
J Gen Virol. 2017 Oct;98(10):2589-2595. doi: 10.1099/jgv.0.000904. Epub 2017 Sep 6.
A novel iridovirus, Cherax quadricarinatus iridovirus (CQIV), was identified from diseased C. quadricarinatus in 2014. This virus is considered as a new threat to crustacean aquaculture because it is lethal to both peneaid shrimp and crayfish. Here, we determined the complete genome sequence of CQIV. The double-stranded DNA genome is 165 695 bp in length with a G+C content of 34.6 %. A total of 178 open reading frames (ORFs) have been predicted, encoding hypothetical proteins ranging from 50 to 1327 amino acids. Forty-seven of these exhibit similarities to proteins of known functions. Phylogenetic analysis based on multiple alignments of conserved proteins shows that CQIV clusters with the members of the family Iridoviridae, but is placed in a distinct clade from all the five known genera. It indicates that CQIV may represent a new genus in the family Iridoviridae, for which we propose the name Cheraxvirus based on the host organism.
2014年，从患病的澳洲淡水龙虾中鉴定出一种新型虹彩病毒——澳洲淡水龙虾虹彩病毒（CQIV）。这种病毒对对虾和小龙虾均具有致死性，因此被视为甲壳类水产养殖的新威胁。在此，我们测定了CQIV的全基因组序列。双链DNA基因组长度为165695 bp，G+C含量为34.6%。共预测出178个开放阅读框（ORF），编码50至1327个氨基酸的假定蛋白。其中47个与已知功能的蛋白具有相似性。基于保守蛋白多序列比对的系统发育分析表明，CQIV与虹彩病毒科成员聚类，但与所有五个已知属处于不同的进化枝。这表明CQIV可能代表虹彩病毒科中的一个新属，我们基于宿主生物为其提议命名为“螯虾病毒属（Cheraxvirus）”。
