Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, 530021, China.
Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, 530021, China.
Fish Shellfish Immunol. 2020 Mar;98:832-842. doi: 10.1016/j.fsi.2019.11.041. Epub 2019 Nov 21.
Cherax quadricarinatus is a large-sized, highly fecund, and fast-growing species of freshwater crayfish, and has become one of the world's most intensely studied crustaceans. Decapod iridescent virus 1 (DIV1), a newly described species in the family Iridoviridae, is known to infect various crustaceans, including C. quadricarinatus, and may pose a new threat in the shrimp-farming industry. The present study performed de novo transcriptome sequencing of C. quadricarinatus hepatopancreas during DIV1 infection. A total of 114,784 transcripts and 56,418 genes were obtained; 1070 genes were upregulated and 775 genes were downregulated when compared with the uninfected samples (controls). Three pattern recognition receptor genes (fibrinogen-related protein, C-type lectin, and beta-1,3-glucan-binding protein) were upregulated during DIV1 infection. Among the top-30 upregulated unigenes, 9 unigenes were identified as vitellogenin (Vg) genes, and the top-3 upregulated unigenes were identified as involved in Vg lipid transport, lipid localization, and lipid transporter activity, which were all significantly over-representative GO terms in the GO enrichment analysis of total and upregulated differentially expressed genes (DEGs). Many genes associated with Jak-STAT signaling pathway, Endocytosis, Phagosome, MAPK signaling pathway, Apoptosis and Lysosome were positively modified after DIV1 infection. The predicted protein-protein interaction (PPI) analysis showed NF1 and TUBA, CRM1 and TUBB were involved in protein interactions. This research showed that DIV1 infection has a significant impact on the transcriptome profile of C. quadricarinatus hepatopancreas, and the results enhance our understanding of virus-host interactions. Furthermore, the high number of transcripts generated in the present study will provide information for identifying novel genes in the absence of a full C. quadricarinatus genome sequence.
拟穴青蟹是一种大型、高繁殖力和快速生长的淡水小龙虾，已成为世界上研究最多的甲壳类动物之一。节肢动物虹彩病毒 1（DIV1）是虹彩病毒科的一个新描述物种，已知感染各种甲壳类动物，包括拟穴青蟹，并可能对虾养殖业构成新的威胁。本研究对感染 DIV1 的拟穴青蟹肝胰腺进行了从头转录组测序。共获得 114784 条转录本和 56418 个基因；与未感染样本（对照）相比，有 1070 个基因上调，775 个基因下调。在 DIV1 感染过程中，三种模式识别受体基因（血纤维蛋白原相关蛋白、C 型凝集素和β-1,3-葡聚糖结合蛋白）上调。在 top-30 上调的 unigenes 中，有 9 个 unigenes被鉴定为卵黄原蛋白（Vg）基因，top-3 上调的 unigenes被鉴定为参与 Vg 脂质转运、脂质定位和脂质转运蛋白活性，这些 unigenes在总差异表达基因（DEGs）和上调 DEGs 的 GO 富集分析中均为显著过代表 GO 术语。许多与 Jak-STAT 信号通路、内吞作用、吞噬体、MAPK 信号通路、细胞凋亡和溶酶体相关的基因在感染 DIV1 后被正向修饰。预测的蛋白质-蛋白质相互作用（PPI）分析显示 NF1 和 TUBA、CRM1 和 TUBB 参与了蛋白质相互作用。本研究表明，DIV1 感染对拟穴青蟹肝胰腺的转录组图谱有显著影响，研究结果增强了我们对病毒-宿主相互作用的理解。此外，本研究中产生的大量转录本将为在缺乏拟穴青蟹完整基因组序列的情况下识别新基因提供信息。
