Rasmussen Louise H, Højholt Katrine, Dargis Rimtas, Christensen Jens Jørgen, Skovgaard Ole, Justesen Ulrik S, Rosenvinge Flemming S, Moser Claus, Lukjancenko Oksana, Rasmussen Simon, Nielsen Xiaohui C
Department of Clinical Microbiology, Slagelse Hospital, Ingemannsvej 46, 4200 Slagelse, Denmark.
Department of Science and Environment, Roskilde University, Universitetsvej 1, 4000 Roskilde, Denmark.
J Med Microbiol. 2017 Sep;66(9):1316-1323. doi: 10.1099/jmm.0.000573.
and belong to the Mitis group, which are mostly commensals in the human oral cavity. Even though and are oral commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of the Mitis group has embedded the species and into the species as subspecies. In this study, the distribution of virulence factors that contribute to bacterial immune evasion, colonization and adhesion was assessed in clinical strains of (subsp. , subsp. and subsp. ) and Forty clinical (subsp. , subsp. dentisani and subsp. ) and genomes were annotated with the pipeline PanFunPro and aligned against the VFDB database for assessment of virulence factors. Three homologues of , and , encoding adhesion proteins, were present in all strains. Seven homologues of and , of importance regarding survival in blood and modulation of the human immune system, were variously present in the genomes. Few subspecies specific differences were observed. homologues were identified in subsp. , whereas homologues were identified in subsp. and subsp. Differences in the presence of virulence factors among the three subspecies were observed. The virulence gene profiles of the 40 and (subsp. , subsp. and subsp. ) contribute with important new knowledge regarding these species and new subspecies.
且属于轻链菌属，它们大多是人类口腔中的共生菌。尽管和是口腔共生菌，但它们可能成为引起感染性心内膜炎的机会致病菌。最近对轻链菌属进行的分类学重新评估已将和物种归入物种作为亚种。在本研究中，在（亚种、亚种和亚种）和的临床菌株中评估了有助于细菌免疫逃避、定植和黏附的毒力因子分布。四十个临床（亚种、亚种和亚种）和基因组用PanFunPro流程进行注释，并与VFDB数据库比对以评估毒力因子。所有菌株中均存在编码黏附蛋白的、和的三个同源物。对在血液中存活和调节人类免疫系统很重要的和的七个同源物在基因组中的存在情况各不相同。观察到很少有亚种特异性差异。在亚种中鉴定出同源物，而在亚种和亚种中鉴定出同源物。观察到三个亚种中毒力因子存在的差异。40个和（亚种、亚种和亚种）的毒力基因谱为这些物种和新亚种提供了重要的新知识。
