Sverdlov E D, Broude N E, Sverdlov V E, Monastyrskaya G S, Grishin A V, Petrukhin K E, Akopyanz N S, Modyanov N N
FEBS Lett. 1987 Aug 31;221(1):129-33. doi: 10.1016/0014-5793(87)80366-6.
Intra-individual tissue-specific restriction fragment length polymorphism (RFLP) has been demonstrated in DNA isolated from different mammalian tissues using cDNAs of alpha- and beta-subunits of Na+,K+-ATPase as hybridization probes. We propose that the RFLPs could result from gene rearrangements in the gene loci for the alpha- and beta-subunits of Na+,K+-ATPase. The changes in restriction patterns have been shown to occur during embryonic development and tumor formation. In addition, the tissue specificity of the expression of different genes of the family of Na+,K+-ATPase genes and their low expression in tumor cells have been demonstrated.
利用钠钾ATP酶α和β亚基的cDNA作为杂交探针，已在从不同哺乳动物组织分离的DNA中证实了个体内组织特异性限制性片段长度多态性（RFLP）。我们认为，RFLP可能是由钠钾ATP酶α和β亚基基因位点的基因重排引起的。已证明限制性图谱的变化发生在胚胎发育和肿瘤形成过程中。此外，还证实了钠钾ATP酶基因家族不同基因表达的组织特异性及其在肿瘤细胞中的低表达。
