Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research "Campus Mare Nostrum", University of Murcia, Murcia, Spain.
Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain.
Sci Rep. 2017 Sep 5;7(1):10505. doi: 10.1038/s41598-017-10989-5.
The oil overlay micro-drop system is widely used for cultures of mammalian gametes and embryos. We evaluated hereby the effects of two unaltered commercial oils- Sigma mineral oil (S-MO) and Nidoil paraffin oil (N-PO)-on in vitro embryo production (IVP) outcomes using a pig model. The results showed that while either oil apparently did not affect oocyte maturation and fertilization rates, S-MO negatively affected embryo cleavage rates, blastocyst formation rates, and, consequently, total blastocyst efficiency of the system. No differences in the oxidation state were found between the oils or culture media incubated under S-MO or N-PO. Although both oils slightly differed in elemental composition, there were no differences in the concentrations of elements between fresh media and media incubated under oils. By contrast, we demonstrated clear oil-type differences in both the composition of volatile organic compounds (VOC) and the transfer of some of these VOC´s (straight-chain alkanes and pentanal and 1,3-diethyl benzene) to the culture medium, which could have influenced embryonic development.
油覆盖微滴系统广泛用于哺乳动物配子和胚胎的培养。我们使用猪模型评估了两种未改变的商业油（Sigma 矿物油[S-MO]和 Nidoil 石蜡油[N-PO]）对体外胚胎生产（IVP）结果的影响。结果表明，尽管两种油显然都不会影响卵母细胞成熟和受精率，但 S-MO 会降低胚胎卵裂率、囊胚形成率，从而降低系统的总囊胚效率。在 S-MO 或 N-PO 孵育下，油和培养基之间的氧化状态没有差异。尽管两种油的元素组成略有不同，但新鲜培养基和油孵育培养基之间的元素浓度没有差异。相比之下，我们证明了挥发性有机化合物（VOC）的组成以及其中一些 VOC（直链烷烃、戊醛和 1,3-二乙基苯）向培养基转移方面存在明显的油类型差异，这可能影响了胚胎发育。
