Centro de Medicina Reprodutiva - ORIGEN, Belo Horizonte, MG, Brazil.
JBRA Assist Reprod. 2023 Mar 30;27(1):35-40. doi: 10.5935/1518-0557.20210123.
The objective of our study was to compare the osmolality in sequential and single step culture media, used for in vitro human embryo culture, covered with mineral oil and paraffin, in dry and humid incubators.
We performed a prospective observational study. A total of 120 Petri dishes, with 960 droplets of culture media, were evaluated. Each dish was prepared with 4 droplets of single step medium and sequential medium. Sixty dishes were covered with mineral oil and 60 with paraffin oil. Half were incubated in a dry incubator and half in a humid. Osmolality was measured on days 1, 3, 5, 7. ANOVA test was performed for statistical analysis.
Osmolality results for single step and sequential medium, that were covered with both mineral and paraffin oil and placed in the dry incubator, significantly increased throughout the study time (D7>D5>D3). In the humid incubator, the results were similar for all periods. Osmolality was significantly lower in humid incubator, in all periods, when droplets were covered with both oils. When both culture media were placed in the humid incubator, no variation was detected, using both oils. However, when single step medium was placed in the dry incubator, covered with mineral oil, we observed a higher osmolality than the covered with paraffin oil.
TWe can conclude that humid incubator is better for maintaining osmolality and paraffin oil protect single step media from evaporation in dry incubator.
我们的研究目的是比较覆盖矿物油和石蜡的用于体外人类胚胎培养的连续和一步法培养基在干燥和加湿孵育器中的渗透压。
我们进行了一项前瞻性观察性研究。共评估了 120 个培养皿，共 960 滴培养介质。每个培养皿用 4 滴一步法培养基和连续法培养基制备。60 个培养皿用矿物油覆盖，60 个用石蜡油覆盖。一半在干燥孵育器中孵育，另一半在加湿孵育器中孵育。在第 1、3、5、7 天测量渗透压。采用方差分析进行统计学分析。
在整个研究期间，覆盖矿物油和石蜡油且置于干燥孵育器中的一步法和连续法培养基的渗透压结果均显著升高（D7>D5>D3）。在加湿孵育器中，所有时期的结果均相似。在所有时期，当滴度覆盖两种油时，加湿孵育器中的渗透压显著较低。当两种培养基均置于加湿孵育器中时，使用两种油均未检测到变化。然而，当一步法培养基置于干燥孵育器并用矿物油覆盖时，我们观察到比用石蜡油覆盖时的渗透压更高。
我们可以得出结论，加湿孵育器更有利于维持渗透压，并且石蜡油可防止干燥孵育器中一步法培养基蒸发。
