Hoogstins Charlotte Es, Weixler Benjamin, Boogerd Leonora Sf, Hoppener Diederik J, Prevoo Hendrica Ajm, Sier Cornelis Fm, Burger Jacobus Wa, Verhoef Cornelis, Bhairosingh Shadvhi, Farina Sarasqueta Arantza, Burggraaf Jacobus, Vahrmeijer Alexander L
Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands.
Department of Surgical Oncology, Erasmus MC Cancer Institute, Rotterdam, The Netherlands.
Biomark Cancer. 2017 Aug 28;9:1179299X17728254. doi: 10.1177/1179299X17728254. eCollection 2017.
Peritoneal metastasis (PM) occurs in about 10% of patients with colorectal cancer (CRC). Fluorescence imaging can enhance contrast between cancerous and benign tissue, enabling the surgeon to clearly visualize PM during cytoreductive surgery. This study assessed the suitability of different biomarkers as potential targets for tumor-targeted imaging of PM of CRC. Tissue samples from primary tumor and PM from patients with CRC were obtained from the pathology archives and immunohistochemical stainings were performed. Overexpression of the epithelial cell adhesion molecule (EpCAM) and carcinoembryonic antigen (CEA) was seen in 100% of PM samples and the expression was strong in >70% of samples. Tyrosine-kinase Met (C-Met) and folate receptor α overexpression was seen in 20% of PM samples. For successful application of tumor-targeted intraoperative fluorescence imaging of PM, biomarkers need to be identified. We demonstrated that both EpCAM and CEA are suitable targets for fluorescence imaging of PM in patients with CRC.
腹膜转移（PM）发生于约10%的结直肠癌（CRC）患者中。荧光成像可增强癌组织与良性组织之间的对比度，使外科医生在减瘤手术期间能够清晰地可视化腹膜转移。本研究评估了不同生物标志物作为CRC腹膜转移肿瘤靶向成像潜在靶点的适用性。从病理档案中获取CRC患者原发性肿瘤和腹膜转移的组织样本，并进行免疫组织化学染色。100%的腹膜转移样本中可见上皮细胞粘附分子（EpCAM）和癌胚抗原（CEA）过表达，且>70%的样本中表达强烈。20%的腹膜转移样本中可见酪氨酸激酶Met（C-Met）和叶酸受体α过表达。为成功应用腹膜转移的肿瘤靶向术中荧光成像，需要鉴定生物标志物。我们证明，EpCAM和CEA都是CRC患者腹膜转移荧光成像的合适靶点。
