Department of Respiratory Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama, 701-0192, Japan.
Neurology, Respirology, Endocrinology, and Metabolism, Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Lung. 2017 Dec;195(6):707-712. doi: 10.1007/s00408-017-0048-8. Epub 2017 Sep 5.
Interleukin (IL)-25 and IL-33 induce IL-5 production by various types of cells, such as type 2 helper T (Th2) cells and type 2 innate lymphoid cells. The number of Th2 cells and concentration of IL-5 in the bronchoalveolar lavage fluid (BALF) are increased in patients with eosinophilic pneumonia (EP). To examine the contribution of IL-25 and IL-33 to eosinophilic inflammation of the lung in humans, we evaluated IL-5, IL-25 and IL-33 levels in the BALF of patients with EP.
IL-5, IL-25, and IL-33 concentrations in the BALF were measured by enzyme-linked immunosorbent assay in patients with acute eosinophilic pneumonia (AEP), chronic eosinophilic pneumonia (CEP), idiopathic pulmonary fibrosis (IPF), and sarcoidosis.
The absolute number of eosinophils, and IL-5 levels, but not IL-33 levels, in the BALF were significantly higher in patients with EP than in patients with IPF and sarcoidosis. IL-25 levels in the BALF were significantly higher in patients with CEP, but not in patients with AEP, than in patients with IPF and sarcoidosis. The absolute number of eosinophils was significantly correlated with the IL-5 concentration in the BALF of patients with EP. IL-5 concentrations were significantly correlated with IL-25 concentrations in the BALF of patients with CEP, but not in patients with AEP. IL-5 levels were not correlated with IL-33 levels in the BALF of patients with EP.
Our findings suggest that IL-25 plays an important role via IL-5 in eosinophilic lung inflammation in patients with CEP.
白细胞介素（IL）-25 和 IL-33 可诱导多种类型的细胞（如 2 型辅助 T（Th2）细胞和 2 型先天淋巴样细胞）产生 IL-5。嗜酸性肺炎（EP）患者的 Th2 细胞数量和支气管肺泡灌洗液（BALF）中 IL-5 浓度增加。为了研究 IL-25 和 IL-33 对人类肺部嗜酸性炎症的贡献，我们评估了 EP 患者 BALF 中的 IL-5、IL-25 和 IL-33 水平。
通过酶联免疫吸附试验测定急性嗜酸性肺炎（AEP）、慢性嗜酸性肺炎（CEP）、特发性肺纤维化（IPF）和结节病患者 BALF 中的 IL-5、IL-25 和 IL-33 浓度。
与 IPF 和结节病患者相比，EP 患者 BALF 中的嗜酸性粒细胞绝对数和 IL-5 水平更高，但 IL-33 水平没有差异。CEP 患者 BALF 中的 IL-25 水平显著高于 IPF 和结节病患者，但 AEP 患者除外。EP 患者 BALF 中的嗜酸性粒细胞绝对数与 IL-5 浓度显著相关。CEP 患者 BALF 中的 IL-5 浓度与 IL-25 浓度显著相关，但 AEP 患者除外。EP 患者 BALF 中的 IL-5 水平与 IL-33 水平无相关性。
我们的发现表明，IL-25 通过 IL-5 在 CEP 患者的嗜酸性肺部炎症中发挥重要作用。
