缺乏γ-谷氨酰转肽酶的大肠杆菌K-12突变体的分离、基因定位及特性分析
Isolation, genetic mapping, and characterization of Escherichia coli K-12 mutants lacking gamma-glutamyltranspeptidase.
作者信息
Suzuki H, Kumagai H, Tochikura T
出版信息
J Bacteriol. 1987 Sep;169(9):3926-31. doi: 10.1128/jb.169.9.3926-3931.1987.
Abstract
Escherichia coli K-12 mutants lacking gamma-glutamyltranspeptidase (EC 2.3.2.2) were isolated after mutagenesis of cells with ethyl methanesulfonate. They lost the enzyme activity to different extents. The mutations of two mutants that had lost the enzyme activity completely were mapped at 76 min of the E. coli K-12 linkage map. These mutations made the cells neither nutrient requiring nor cold sensitive. The mutants leaked much more glutathione into the medium than the wild type. We propose the symbol ggt for these mutations.
摘要
用甲磺酸乙酯对细胞进行诱变后，分离出了缺乏γ-谷氨酰转肽酶（EC 2.3.2.2）的大肠杆菌K-12突变体。它们的酶活性有不同程度的丧失。两个完全丧失酶活性的突变体的突变位点在大肠杆菌K-12连锁图谱的76分钟处。这些突变使细胞既不依赖营养物质也不具有冷敏感性。与野生型相比，这些突变体向培养基中泄漏的谷胱甘肽要多得多。我们提议用符号ggt表示这些突变。
相似文献
1
Isolation, genetic mapping, and characterization of Escherichia coli K-12 mutants lacking gamma-glutamyltranspeptidase.缺乏γ-谷氨酰转肽酶的大肠杆菌K-12突变体的分离、基因定位及特性分析
J Bacteriol. 1987 Sep;169(9):3926-31. doi: 10.1128/jb.169.9.3926-3931.1987.
2
Molecular cloning of Escherichia coli K-12 ggt and rapid isolation of gamma-glutamyltranspeptidase.
Biochem Biophys Res Commun. 1988 Jan 15;150(1):33-8. doi: 10.1016/0006-291x(88)90482-2.
3
Effect of site-directed mutations on processing and activity of gamma-glutamyltranspeptidase of Escherichia coli K-12.定点突变对大肠杆菌K-12γ-谷氨酰转肽酶加工及活性的影响
J Biochem. 1995 Jul;118(1):75-80. doi: 10.1093/oxfordjournals.jbchem.a124894.
4
DNA sequence of the Escherichia coli K-12 gamma-glutamyltranspeptidase gene, ggt.大肠杆菌K-12γ-谷氨酰转肽酶基因ggt的DNA序列
J Bacteriol. 1989 Sep;171(9):5169-72. doi: 10.1128/jb.171.9.5169-5172.1989.
5
Escherichia coli gamma-glutamyltranspeptidase mutants deficient in processing to subunits.
Biochem Biophys Res Commun. 1992 Nov 30;189(1):173-8. doi: 10.1016/0006-291x(92)91540-7.
6
[Gamma-glutamyltranspeptidase from Escherichia coli K-12].[来自大肠杆菌K-12的γ-谷氨酰转肽酶]
Seikagaku. 1989 Jun;61(6):491-6.
7
Residues Arg114 and Arg337 are critical for the proper function of Escherichia coli gamma-glutamyltranspeptidase.残基精氨酸114和精氨酸337对大肠杆菌γ-谷氨酰转肽酶的正常功能至关重要。
Biochem Biophys Res Commun. 2008 Feb 8;366(2):294-300. doi: 10.1016/j.bbrc.2007.11.063. Epub 2007 Nov 26.
8
Analysis of low temperature inducible mechanism of gamma-glutamyltranspeptidase of Escherichia coli K-12.大肠杆菌K-12γ-谷氨酰转肽酶低温诱导机制分析
Biosci Biotechnol Biochem. 1997 Jan;61(1):34-9. doi: 10.1271/bbb.61.34.
9
Site-directed mutagenesis of conserved Thr407, Asp433 and Met464 residues in small subunit of Escherichia coli gamma-glutamyltranspeptidase.大肠杆菌γ-谷氨酰转肽酶小亚基中保守的苏氨酸407、天冬氨酸433和甲硫氨酸464残基的定点诱变
Indian J Biochem Biophys. 2007 Aug;44(4):197-203.
10
Isolation and mapping of glutathione reductase-negative mutants of Escherichia coli K12.大肠杆菌K12谷胱甘肽还原酶阴性突变体的分离与定位
J Gen Microbiol. 1982 Jul;128(7):1631-4. doi: 10.1099/00221287-128-7-1631.
引用本文的文献
1
Bacterial Gamma-Glutamyl Transpeptidase, an Emerging Biocatalyst: Insights Into Structure-Function Relationship and Its Biotechnological Applications.细菌γ-谷氨酰转肽酶：一种新兴的生物催化剂——对结构-功能关系及其生物技术应用的见解
Front Microbiol. 2021 Apr 9;12:641251. doi: 10.3389/fmicb.2021.641251. eCollection 2021.
2
Bacterial γ-glutamyltranspeptidases, physiological function, structure, catalytic mechanism and application.细菌γ-谷氨酰转肽酶、生理功能、结构、催化机制及应用
Proc Jpn Acad Ser B Phys Biol Sci. 2020;96(9):440-469. doi: 10.2183/pjab.96.033.
3
Purification and Characterization of Glutathione Binding Protein GsiB from .从 中纯化和表征谷胱甘肽结合蛋白 GsiB。
Biomed Res Int. 2018 Nov 1;2018:3429569. doi: 10.1155/2018/3429569. eCollection 2018.
4
Purification and Characterization of an ATPase GsiA from .来自……的一种ATP酶GsiA的纯化与特性分析
Biomed Res Int. 2017;2017:3076091. doi: 10.1155/2017/3076091. Epub 2017 Jun 12.
5
Anaerobic Cysteine Degradation and Potential Metabolic Coordination in Salmonella enterica and Escherichia coli.肠炎沙门氏菌和大肠杆菌中的厌氧半胱氨酸降解及潜在的代谢协调
J Bacteriol. 2017 Jul 25;199(16). doi: 10.1128/JB.00117-17. Print 2017 Aug 15.
6
Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion.通过 GFP 融合优化大肠杆菌 K-12 中 ATP 结合基因 gsiA 的表达和纯化。
Genet Mol Biol. 2011 Oct;34(4):661-8. doi: 10.1590/S1415-47572011005000043. Epub 2011 Oct 1.
7
A novel putrescine importer required for type 1 pili-driven surface motility induced by extracellular putrescine in Escherichia coli K-12.一种新型腐胺转运蛋白，是大肠杆菌 K-12 中由细胞外腐胺诱导的 1 型菌毛驱动的表面运动所必需的。
J Biol Chem. 2011 Mar 25;286(12):10185-92. doi: 10.1074/jbc.M110.176032. Epub 2011 Jan 25.
8
A putrescine-inducible pathway comprising PuuE-YneI in which gamma-aminobutyrate is degraded into succinate in Escherichia coli K-12.在大肠杆菌 K-12 中，腐胺诱导的途径包括 PuuE-YneI，在此途径中γ-氨基丁酸被降解为琥珀酸。
J Bacteriol. 2010 Sep;192(18):4582-91. doi: 10.1128/JB.00308-10. Epub 2010 Jul 16.
9
The putrescine Importer PuuP of Escherichia coli K-12.大肠杆菌K-12的腐胺导入蛋白PuuP
J Bacteriol. 2009 Apr;191(8):2776-82. doi: 10.1128/JB.01314-08. Epub 2009 Jan 30.
10
The yliA, -B, -C, and -D genes of Escherichia coli K-12 encode a novel glutathione importer with an ATP-binding cassette.大肠杆菌K-12的yliA、-B、-C和-D基因编码一种带有ATP结合盒的新型谷胱甘肽转运体。
J Bacteriol. 2005 Sep;187(17):5861-7. doi: 10.1128/JB.187.17.5861-5867.2005.
本文引用的文献
1
Normal amino acid uptake by cultured human fibroblasts does not require gamma-glutamyl transpeptidase.培养的人成纤维细胞对正常氨基酸的摄取不需要γ-谷氨酰转肽酶。
Biochem Biophys Res Commun. 1976 Dec 20;73(4):997-1002. doi: 10.1016/0006-291x(76)90221-7.
2
ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.大肠杆菌中L-α-甘油磷酸的主动运输
J Biol Chem. 1964 Sep;239:3098-105.
3
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
4
Glutathione breakdown and transpeptidation reactions in Proteus vulgaris.普通变形杆菌中的谷胱甘肽分解及转肽反应
Nature. 1954 Sep 11;174(4428):516-7. doi: 10.1038/174516b0.
5
Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion.凝胶中的抗原-抗体反应。IV. 扩散协调系统中的反应类型。
Acta Pathol Microbiol Scand. 1953;32(2):230-40.
6
The role of glutathione in amino-acid absorption. Lack of correlation between glutathione turnover and amino-acid absorption by the yeast Candida utilis.谷胱甘肽在氨基酸吸收中的作用。产朊假丝酵母中谷胱甘肽周转与氨基酸吸收之间缺乏相关性。
Biochem J. 1981 Jan 15;194(1):63-70. doi: 10.1042/bj1940063.
7
Positive regulatory gene for temperature-controlled proteins in Escherichia coli.大肠杆菌中温度调控蛋白的正调控基因。
Biochem Biophys Res Commun. 1981 May 29;100(2):894-900. doi: 10.1016/s0006-291x(81)80257-4.
8
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
9
Purification and characterization of glutathione synthetase from Escherichia coli B.来自大肠杆菌B的谷胱甘肽合成酶的纯化与特性分析
J Appl Biochem. 1983 Jun;5(3):210-8.
10
Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
Zotero 插件