谷胱甘肽在氨基酸吸收中的作用。产朊假丝酵母中谷胱甘肽周转与氨基酸吸收之间缺乏相关性。
The role of glutathione in amino-acid absorption. Lack of correlation between glutathione turnover and amino-acid absorption by the yeast Candida utilis.
作者信息
Robins R J, Davies D D
出版信息
Biochem J. 1981 Jan 15;194(1):63-70. doi: 10.1042/bj1940063.
Abstract
The rate of degradation of glutathione has been determined in the yeast Candida utilis by using a method that minimizes the effect of amino-acid recycling. When yeast are grown in amino-acid-free medium, the half-life of glutathione was found to be 230 min. C. utilis was also found to absorb various L-amino acids rapidly without producing any significant decrease in the half-life of glutathione. While the gamma-glutamyl cycle is thus operating in C. utilis, the rate of degradation of glutathione is found to be 100 times too slow for the cycle to be mediating the transport of these amino acids.
摘要
通过使用一种能将氨基酸循环影响降至最低的方法，测定了产朊假丝酵母中谷胱甘肽的降解速率。当酵母在无氨基酸培养基中生长时，发现谷胱甘肽的半衰期为230分钟。还发现产朊假丝酵母能迅速吸收各种L-氨基酸，而谷胱甘肽的半衰期没有任何显著降低。因此，虽然γ-谷氨酰循环在产朊假丝酵母中起作用，但发现谷胱甘肽的降解速率比该循环介导这些氨基酸转运的速率慢100倍。
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本文引用的文献
1
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Plant Physiol. 1978 Jan;61(1):54-8. doi: 10.1104/pp.61.1.54.
2
Enzymic transpeptidation reactions involving gamma-glutamyl peptides and alpha-amino-acyl peptides.涉及γ-谷氨酰肽和α-氨基酰肽的酶促转肽反应。
Biochem J. 1952 Apr;51(1):25-35. doi: 10.1042/bj0510025.
3
The reaction of glutathione with amino acids and related compounds as catalyzed by gamma-glutamyl transpeptidase.γ-谷氨酰转肽酶催化的谷胱甘肽与氨基酸及相关化合物的反应。
J Biol Chem. 1959 Mar;234(3):577-82.
4
The kinetics of the gamma-glutamyl cycle-mediated uptake of amino acids: considerations explaining the bifurcation of the gamma-glutamyl cycle.γ-谷氨酰循环介导的氨基酸摄取动力学：解释γ-谷氨酰循环分支的相关因素
FEBS Lett. 1980 Feb 11;110(2):192-4. doi: 10.1016/0014-5793(80)80070-6.
5
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FEBS Lett. 1980 Mar 10;111(2):432. doi: 10.1016/0014-5793(80)80843-x.
6
Glutathione metabolism in relation to the amino-acid permeation systems of the yeast Saccharomyces cerevisiae. Occurrence of gamma-glutamyltranspeptidase: its regulation and the effects of permeation mutations on the enzyme cellular level.酿酒酵母谷胱甘肽代谢与氨基酸通透系统的关系。γ-谷氨酰转肽酶的存在：其调控以及通透突变对酶细胞水平的影响。
Eur J Biochem. 1980 Feb;104(1):119-23. doi: 10.1111/j.1432-1033.1980.tb04407.x.
7
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8
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10
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