一种由Cre依赖性FLEx开关控制的、用于通过白喉毒素亚基A进行细胞消融的新型小鼠品系。
A new mouse line for cell ablation by diphtheria toxin subunit A controlled by a Cre-dependent FLEx switch.
作者信息
Plummer Nicholas W, Ungewitter Erica K, Smith Kathleen G, Yao Humphrey H-C, Jensen Patricia
机构信息
Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina, 27709, USA.
Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina, 27709, USA.
出版信息
Genesis. 2017 Oct;55(10). doi: 10.1002/dvg.23067. Epub 2017 Sep 19.
Recombinase responsive mouse lines expressing diphtheria toxin subunit A (DTA) are well established tools for targeted ablation of genetically defined cell populations. Here we describe a new knock-in allele at the Gt(Rosa)26Sor locus that retains the best features of previously described DTA alleles-including a CAG promoter, attenuated mutant DTA cDNA, and ubiquitous EGFP labeling-with the addition of a Cre-dependent FLEx switch for tight control of expression. The FLEx switch consists of two pairs of antiparallel lox sites requiring Cre-mediated recombination for inversion of the DTA to the proper orientation for transcription. We demonstrate its utility by Cre-dependent ablation of both a broad domain in the embryonic nervous system and a discrete population of cells in the fetal gonads. We conclude that this new DTA line is useful for targeted ablation of genetically-defined cell populations.
表达白喉毒素A亚基（DTA）的重组酶反应性小鼠品系是用于靶向消融基因定义细胞群体的成熟工具。在此，我们描述了一个位于Gt(Rosa)26Sor位点的新敲入等位基因，它保留了先前描述的DTA等位基因的最佳特性，包括CAG启动子、减毒突变DTA cDNA和普遍的EGFP标记，并增加了一个Cre依赖性FLEx开关以严格控制表达。FLEx开关由两对反平行的lox位点组成，需要Cre介导的重组才能将DTA反转到正确的转录方向。我们通过在胚胎神经系统中的广泛区域和胎儿性腺中的离散细胞群体进行Cre依赖性消融来证明其效用。我们得出结论，这个新的DTA品系可用于靶向消融基因定义的细胞群体。
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