Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N Broad Street, Philadelphia, PA, 19140, USA.
Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, 70433, USA.
Cell Mol Life Sci. 2019 Dec;76(23):4725-4743. doi: 10.1007/s00018-019-03243-w. Epub 2019 Jul 29.
Targeted cell ablation is a powerful approach for studying the role of specific cell populations in a variety of organotypic functions, including cell differentiation, and organ generation and regeneration. Emerging tools for permanently or conditionally ablating targeted cell populations and transiently inhibiting neuronal activities exhibit a diversity of application and utility. Each tool has distinct features, and none can be universally applied to study different cell types in various tissue compartments. Although these tools have been developed for over 30 years, they require additional improvement. Currently, there is no consensus on how to select the tools to answer the specific scientific questions of interest. Selecting the appropriate cell ablation technique to study the function of a targeted cell population is less straightforward than selecting the method to study a gene's functions. In this review, we discuss the features of the various tools for targeted cell ablation and provide recommendations for optimal application of specific approaches.
靶向细胞消融是一种强大的方法，可用于研究特定细胞群体在各种器官功能中的作用，包括细胞分化、器官生成和再生。用于永久或条件性消融靶向细胞群体和瞬时抑制神经元活动的新兴工具具有多种应用和实用性。每种工具都具有不同的特点，而且没有一种工具可以普遍应用于研究不同组织隔室中的不同细胞类型。尽管这些工具已经开发了 30 多年，但它们仍需要进一步改进。目前，对于如何选择工具来回答特定的科学问题，尚无共识。选择适当的细胞消融技术来研究靶向细胞群体的功能并不像选择研究基因功能的方法那样简单。在这篇综述中，我们讨论了各种靶向细胞消融工具的特点，并就特定方法的最佳应用提供了建议。
