Li Meng-Meng, Guo Qiao-Sheng, Shi Hong-Zhuan, Wang Jia, Dai Dao-Xin, Yan Shi-Meng, Qi Heng-Ji, Lu Xin, Liu Li-Tao
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Zhongguo Zhong Yao Za Zhi. 2016 Mar;41(6):995-1000. doi: 10.4268/cjcmm20160604.
The effects of stocking density and exchanging water frequency on growth, digestive enzyme activity, anti-oxidative enzyme and inner quality of Whitmania pigra Whitman were evaluated with corresponding measures. The results showed that the eventual biomass, specific growth rate, gained weight rate, activities of amylase, lipase, protease, SOD, CAT, and ALP correlated positively with stocking density and negatively with exchanging water frequency (P<0.05). Exchanging water frequency had negative correlation with ammonia nitrogen, nitrite, and hydrogen sulfide while revealed positive correlation with dissolved oxygen in the water. Stocking density and exchanging water frequency showed no significant effects on the contents of moisture, total ash, and acid-insoluble ash. It suggested that the optimum stocking density was 7.5 million per hectare and the appropriate exchanging water interval was 72 h.
通过相应措施评估了放养密度和换水频率对宽体金线蛭生长、消化酶活性、抗氧化酶及内在品质的影响。结果表明，最终生物量、特定生长率、增重率、淀粉酶、脂肪酶、蛋白酶、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和碱性磷酸酶（ALP）的活性与放养密度呈正相关，与换水频率呈负相关（P<0.05）。换水频率与氨氮、亚硝酸盐和硫化氢呈负相关，而与水中溶解氧呈正相关。放养密度和换水频率对水分、总灰分和酸不溶性灰分的含量无显著影响。结果表明，最佳放养密度为每公顷750万只，适宜的换水间隔为72小时。
