Wu Bin, Guo Qiao-Sheng, Shi Hong-Zhuan, Shi Guo-Wei, Yan Shi-Meng, Wu Man-Jun, Wang Shuai, Lu Xin, Chen Pan-Pan, Yan Xiao-Lu
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Zhongguo Zhong Yao Za Zhi. 2018 Sep;43(18):3611-3617. doi: 10.19540/j.cnki.cjcmm.20180703.006.
To explore the effect and mechanism of Astragali Radix on growth and immunity of Whitmania pigra, 0, 0.01%, 0.03%, 0.05%, 0.07%, 0.09% of Astragali Radix were added to the daily feeding of Wh. pigra. After 60 days of feeding, the growth performance, activities of digestive enzyme and anti-reverse enzyme, inner quality, the expression levels of GH, IGF-1 and digestive enzyme-related genes were measured. Meanwhile, the effects of heat stress on the living conditions of Wh. pigra were observed and counted, and the expression levels of HSP70 and immune related genes were measured. The results showed that the final weight, weight gain rate, specific growth rate, activities of digestive enzyme and anti-reverse enzyme, the expression levels of GH, IGF-1 and digestive enzyme-related genes in the Astragali Radix group were higher than those in the control group, and with the increase of Astragali Radix concentration, the above-mentioned indexes increased initially and then decreased, and significantly higher in the 0.05% of Astragali Radix group than in the other groups (<0.05). There was no significant difference in the inner quality of Wh. pigra between the Astragali Radix and control groups. The survival rate of Wh. pigra was negatively correlated with heat stress treatment duration. With the prolongation of heat stress treatment duration, the expression levels of HSP70 and immune related genes were increased first and then decreased, and peaked at 24 h. The survival rate and the expression levels of HSP70 and immune related genes in the Astragali Radix group were higher than those in the control group, and was significantly higher in the 0.05% of Astragali Radix group than in the other groups (<0.05). In conclusion, Astragali Radix can increase the activities of digestive enzyme and anti-reverse enzyme, the expression levels of related genes, growth performance, and immunity to heat stress of Wh. pigra. It is suggested to add 0.05% of Astragali Radix in the actual production of Wh. pigra to improve the production profit.
为探讨黄芪对蚂蟥生长和免疫的影响及其作用机制，在蚂蟥的日常投喂中分别添加0、0.01%、0.03%、0.05%、0.07%、0.09%的黄芪。投喂60天后，测定蚂蟥的生长性能、消化酶和抗逆酶活性、内在品质、生长激素（GH）、胰岛素样生长因子-1（IGF-1）及消化酶相关基因的表达水平。同时，观察并统计热应激对蚂蟥生存状况的影响，测定热休克蛋白70（HSP70）和免疫相关基因的表达水平。结果显示，黄芪组蚂蟥的终末体重、增重率、特定生长率、消化酶和抗逆酶活性、GH、IGF-1及消化酶相关基因的表达水平均高于对照组，且随着黄芪浓度的增加，上述指标先升高后降低，其中0.05%黄芪组显著高于其他各组（P<0.05）。黄芪组与对照组蚂蟥的内在品质无显著差异。蚂蟥的存活率与热应激处理时间呈负相关。随着热应激处理时间的延长，HSP70和免疫相关基因的表达水平先升高后降低，在24 h时达到峰值。黄芪组蚂蟥的存活率及HSP70和免疫相关基因的表达水平均高于对照组，且0.05%黄芪组显著高于其他各组（P<0.05）。综上所述，黄芪可提高蚂蟥的消化酶和抗逆酶活性、相关基因的表达水平、生长性能及对热应激的免疫力。建议在蚂蟥实际生产中添加0.05%的黄芪以提高生产效益。
