Shincy J S, Panagal Mani, Jereena Jinu, Vengatagiri Giri Yogesh, Vittalrao Kshirsagar Rahul, Sivakumar Pethanen, Gopinath Vincent, Kumar K M, Sekar Durairaj
Department of Biotechnology, School of Chemical and Biological Sciences, REVA University, Bangalore, India.
Department of Biotechnology, Annai College of Arts and Science, Kumbakonam, Tamil Nadu, India.
Microrna. 2017 Dec 6;6(3):208-212. doi: 10.2174/2211536606666170830120427.
MicroRNAs (miRNAs) are small, highly conserved non-coding RNA molecules involved in the RNA silencing and post-transcriptional regulation of gene expression. miRNAs are well conserved in both plants and animals, and are thought to be a vital and evolutionarily ancient component of gene regulation and also act as oncogenes or tumor suppressors. It is known that Express Sequence Tags (EST) are a short sub-sequence of cDNA sequence, which contain information of condition or tissue specific transcripts (coding and non-coding) of an organism.
In the present study, we have applied the bioinformatics tools to identify miRNA from breast cancer using EST resource. Through bioinformatics approach, the presence of an EST encoding hsa-miR-17- 3p of breast cancer was identified.
Further studies reveal that hsa-miR-17 is confirmed in the breast cancer specific EST sequence among the predicted miRNAs secondary structure. Moreover, miR-17-3p could be responsible for a tumor suppression, which plays a major role in human breast cancer.
Further studies are required to investigate the molecular mechanisms behind miR-17-3p involves in the suppression of breast cancer cells. Interestingly, our target analysis suggesting that all the targets involved in multiple signaling pathways in different cell regulations moreover, we need to have more number of in vitro and in vivo studies that prove miR-17-3p as candidate microRNA for breast cancer cells.
微小RNA（miRNA）是小的、高度保守的非编码RNA分子，参与RNA沉默和基因表达的转录后调控。miRNA在植物和动物中都高度保守，被认为是基因调控中至关重要且在进化上古老的组成部分，同时还可作为癌基因或肿瘤抑制因子。已知表达序列标签（EST）是cDNA序列的短子序列，包含生物体条件或组织特异性转录本（编码和非编码）的信息。
在本研究中，我们应用生物信息学工具利用EST资源从乳腺癌中鉴定miRNA。通过生物信息学方法，在乳腺癌中鉴定出一个编码hsa-miR-17-3p的EST的存在。
进一步研究表明，在预测的miRNA二级结构中，hsa-miR-17在乳腺癌特异性EST序列中得到证实。此外，miR-17-3p可能具有肿瘤抑制作用，在人类乳腺癌中起主要作用。
需要进一步研究来探讨miR-17-3p抑制乳腺癌细胞背后的分子机制。有趣的是，我们的靶标分析表明，所有靶标都参与不同细胞调控中的多种信号通路，此外，我们需要更多的体外和体内研究来证明miR-17-3p是乳腺癌细胞的候选微小RNA。
