Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, P.R. China.
Key Laboratory of Women's Reproductive Health of Zhejiang Province, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, P.R. China.
Medicine (Baltimore). 2023 Aug 25;102(34):e34998. doi: 10.1097/MD.0000000000034998.
Endometrial cancer (EC) is one of the most common gynecological malignancies worldwide. Accumulated evidence has demonstrated exosomes of cancer cells carry microRNAs (miRNAs) to nonmalignant cells to induce metastasis. Our study aimed to find possible biomarkers of EC. Data for miRNA expression related with exosome from EC patients were downloaded from The Cancer Genome Atlas database, and the miRNA expression profiles associated with exosomes of EC were downloaded from the National Center for Biotechnology Information. We used different algorithms to analyze the differential miRNA expression, infer the relative proportion of immune infiltrating cells, predict chemotherapy sensitivity, and comprehensively score each gene set to evaluate the potential biological function changes of different samples. The gene ontology analysis and Kyoto encyclopedia of genome genomics pathway analysis were performed for specific genes. A total of 13 differential miRNAs were identified, of which 4 were up-regulated. The 4 miRNAs, that is hsa-miR-17-3p, hsa-miR-99b-3p, hsa-miR-193a-5p, and hsa-miR-320d, were the hub exosomal miRNAs that were all closely related to the clinic phenotypes and prognosis of patients. This study preliminarily indicates that the 4 hub exosomal miRNAs (hsa-miR-17-3p, hsa-miR-99b-3p, hsa-miR-193a-5p, and hsa-miR-320d) could be used as prognostic biomarkers or therapy targets in EC. Further studies are required to make sure of their real feasibility and values in the EC clinic and the relative research.
子宫内膜癌（EC）是全球最常见的妇科恶性肿瘤之一。大量证据表明，癌细胞来源的外泌体携带 microRNAs（miRNAs）到非恶性细胞中，从而诱导转移。本研究旨在寻找 EC 的可能生物标志物。从癌症基因组图谱数据库中下载与 EC 患者外泌体相关的 miRNA 表达数据，从国家生物技术信息中心下载与 EC 外泌体相关的 miRNA 表达谱。我们使用不同的算法来分析差异表达的 miRNA，推断免疫浸润细胞的相对比例，预测化疗敏感性，并对每个基因集进行全面评分，以评估不同样本的潜在生物学功能变化。对特定基因进行基因本体分析和京都基因与基因组百科全书通路分析。共鉴定出 13 个差异表达的 miRNA，其中 4 个上调。这 4 个 miRNA，即 hsa-miR-17-3p、hsa-miR-99b-3p、hsa-miR-193a-5p 和 hsa-miR-320d，是与患者临床表型和预后密切相关的核心外泌体 miRNA。本研究初步表明，这 4 个核心外泌体 miRNA（hsa-miR-17-3p、hsa-miR-99b-3p、hsa-miR-193a-5p 和 hsa-miR-320d）可作为 EC 的预后生物标志物或治疗靶点。需要进一步的研究来确定它们在 EC 临床和相关研究中的真正可行性和价值。
