女性阴道样本中的 Y 染色体 DNA 作为近期阴道性行为和与男性伴侣使用避孕套的生物标志物：通过异性活动队列研究中的 HPV 感染和传播。
Y Chromosome DNA in Women's Vaginal Samples as a Biomarker of Recent Vaginal Sex and Condom Use With Male Partners in the HPV Infection and Transmission Among Couples Through Heterosexual Activity Cohort Study.
出版信息
Sex Transm Dis. 2018 Jan;45(1):28-34. doi: 10.1097/OLQ.0000000000000688.
BACKGROUND
Y chromosome DNA from male epithelial and sperm cells was detected in vaginal samples after unprotected sex in experimental studies. We assessed the strength of this association in an observational setting to examine the utility of Y chromosome DNA as a biomarker of recent sexual behaviors in epidemiological studies.
METHODS
The HPV (human papillomavirus) Infection and Transmission Among Couples Through Heterosexual Activity cohort study enrolled 502 women attending a university or college in Montréal, Canada, and their male partners from 2005 to 2010. Participants completed self-administered questionnaires. We used real-time polymerase chain reaction to test women's baseline vaginal samples for Y chromosome DNA and assessed which sexual behaviors were independent predictors of Y chromosome DNA positivity and quantity with logistic and negative binomial regression.
RESULTS
Y chromosome DNA positivity decreased from 77% in women in partnerships reporting vaginal sex 0 to 1 day ago to 13% in women in partnerships reporting last vaginal sex of 15 or more days ago (adjusted odds ratio, 0.09; 95% confidence interval, 0.02-0.36). The mean proportion of exfoliated vaginal sample cells with Y chromosome DNA was much lower for women who reported always using condoms (0.01%) than for women who reported never using condoms (2.07%) (adjusted ratio, 26.8; 95% confidence interval, 8.9-80.5). No association was found with reported oral/digital sex frequency or concurrency of partnerships.
CONCLUSIONS
Y chromosome DNA quantity is strongly associated with days since last vaginal sex and lack of condom use in observational settings. Y chromosome DNA quantity may prove useful as a correlate of recent vaginal sex in observational studies lacking data on sexual behavior, such as surveillance studies of human papillomavirus infection prevalence.
背景
在实验研究中，曾在无保护性行为后于阴道样本中检测到来自男性上皮细胞和精子的 Y 染色体 DNA。我们在观察性环境中评估了这种关联的强度，以检验 Y 染色体 DNA 作为流行病学研究中近期性行为生物标志物的效用。
方法
HPV（人乳头瘤病毒）感染和异性活动中伴侣间传播队列研究纳入了 2005 年至 2010 年期间在加拿大蒙特利尔的一所大学或学院就诊的 502 名女性及其男性伴侣。参与者完成了自我管理的问卷。我们使用实时聚合酶链反应检测女性基线阴道样本中的 Y 染色体 DNA，并使用逻辑回归和负二项回归评估哪些性行为是 Y 染色体 DNA 阳性和数量的独立预测因子。
结果
在报告阴道性交 0-1 天前的伴侣中，女性 Y 染色体 DNA 阳性率从 77%降至报告上次阴道性交 15 天或更长时间前的伴侣中的 13%（调整后的优势比，0.09；95%置信区间，0.02-0.36）。报告总是使用避孕套的女性的脱落阴道样本细胞中 Y 染色体 DNA 的平均比例（0.01%）明显低于报告从不使用避孕套的女性（2.07%）（调整比值，26.8；95%置信区间，8.9-80.5）。报告的口交/阴交频率或伴侣关系的同时性与 Y 染色体 DNA 数量无关联。
结论
在观察性环境中，Y 染色体 DNA 数量与上次阴道性交后天数和缺乏避孕套使用密切相关。在缺乏性行为数据的观察性研究中，如人类乳头瘤病毒感染流行率监测研究中，Y 染色体 DNA 数量可能是近期阴道性交的有用相关指标。
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