Lima Jailton Lobo da Costa, Alves Lilian Rodrigues, Paz Jussyêgles Niedja Pereira da, Rabelo Marcelle Aquino, Maciel Maria Amélia Vieira, Morais Marcia Maria Camargo de
Programa de Pós-graduação em Medicina Tropical, Centro de Ciências da Saúde, Universidade Federal de Pernambuco - Recife (PE), Brasil.
Programa de Pós-graduação em Biologia Celular e Molecular Aplicada, Instituto de Ciências Biológicas, Universidade de Pernambuco - Recife (PE), Brasil.
Rev Bras Ter Intensiva. 2017 Jul-Sep;29(3):310-316. doi: 10.5935/0103-507X.20170039. Epub 2017 Sep 4.
To phenotypically evaluate biofilm production by Pseudomonas aeruginosa clinically isolated from patients with ventilator-associated pneumonia.
Twenty clinical isolates of P. aeruginosa were analyzed, 19 of which were from clinical samples of tracheal aspirate, and one was from a bronchoalveolar lavage sample. The evaluation of the capacity of P. aeruginosa to produce biofilm was verified using two techniques, one qualitative and the other quantitative.
The qualitative technique showed that only 15% of the isolates were considered biofilm producers, while the quantitative technique showed that 75% of the isolates were biofilm producers. The biofilm isolates presented the following susceptibility profile: 53.3% were multidrug-resistant, and 46.7% were multidrug-sensitive.
The quantitative technique was more effective than the qualitative technique for the detection of biofilm production. For the bacterial population analyzed, biofilm production was independent of the susceptibility profile of the bacteria, demonstrating that the therapeutic failure could be related to biofilm production, as it prevented the destruction of the bacteria present in this structure, causing complications of pneumonia associated with mechanical ventilation, including extrapulmonary infections, and making it difficult to treat the infection.
对从呼吸机相关性肺炎患者临床分离出的铜绿假单胞菌的生物膜形成进行表型评估。
分析了20株铜绿假单胞菌临床分离株，其中19株来自气管抽吸物临床样本，1株来自支气管肺泡灌洗样本。使用两种技术验证铜绿假单胞菌产生生物膜的能力，一种是定性技术，另一种是定量技术。
定性技术显示只有15%的分离株被认为是生物膜产生菌，而定量技术显示75%的分离株是生物膜产生菌。生物膜分离株呈现以下药敏谱：53.3%为多重耐药，46.7%为多重敏感。
在检测生物膜形成方面，定量技术比定性技术更有效。对于所分析的细菌群体，生物膜形成与细菌的药敏谱无关，这表明治疗失败可能与生物膜形成有关，因为它阻止了存在于该结构中的细菌被破坏，导致与机械通气相关的肺炎并发症，包括肺外感染，并使感染难以治疗。
