Department of Microbiology, Kantipur College of Medical Science, Sitapaila, Kathmandu, Nepal.
Department of Microbiology, Grande International Hospital, Dhapasi, Kathmandu, Nepal.
Ann Clin Microbiol Antimicrob. 2017 Nov 2;16(1):70. doi: 10.1186/s12941-017-0245-6.
Pseudomonas aeruginosa and Acinetobacter spp. are found to be associated with biofilm and metallo-β-lactamase production and are the common causes of serious infections mainly in hospitalized patients. So, the main aims of this study were to determine the rates of biofilm production and metallo beta-lactamase production (MBL) among the strains of Pseudomonas aeruginosa and Acinetobacter spp. isolated from hospitalized patients.
A total of 85 P. aeruginosa isolates and 50 Acinetobacter spp. isolates isolated from different clinical specimens from patients admitted to Shree Birendra Hospital, Kathmandu, Nepal from July 2013 to May 2014 were included in this study. The bacterial isolates were identified with the help of biochemical tests. Modified Kirby-Bauer disc diffusion technique was used for antimicrobial susceptibility testing. Combined disc diffusion technique was used for the detection of MBL production, while Congo red agar method and tube adherence method were used for detection of biofilm production.
Around 16.4% of P. aeruginosa isolates and 22% of the strains of Acinetobacter spp. were metallo β-lactamase producers. Out of 85 P. aeruginosa isolates, 23 (27.05%) were biofilm producers according to tube adherence test while, only 13 (15.29%) were biofilm producers as per Congo red agar method. Similarly, out of 50 Acinetobacter spp. 7 (14%) isolates were biofilm producers on the basis of tube adherence test, while only 5 (10%) were positive for biofilm production by Congo red agar method. Highest rates of susceptibility of P. aeruginosa as well as Acinetobacter spp. were seen toward colistin.
In our study, biofilm production and metallo beta-lactamase production were observed among Pseudomonas aeruginosa and Acinetobacter spp. However, no statistically significant association could be established between biofilm production and metallo beta-lactamase production.
铜绿假单胞菌和不动杆菌属与生物膜和金属β-内酰胺酶的产生有关，是住院患者中严重感染的常见原因。因此，本研究的主要目的是确定从尼泊尔加德满都 Shree Birendra 医院住院患者的不同临床标本中分离的铜绿假单胞菌和不动杆菌属菌株的生物膜产生率和金属β-内酰胺酶（MBL）产生率。
本研究共纳入了 2013 年 7 月至 2014 年 5 月从尼泊尔加德满都 Shree Birendra 医院住院患者的不同临床标本中分离的 85 株铜绿假单胞菌和 50 株不动杆菌属菌株。使用生化试验协助鉴定细菌分离株。采用改良 Kirby-Bauer 纸片扩散法进行抗菌药物敏感性试验。联合纸片扩散法用于检测 MBL 产生，而刚果红琼脂法和管附着法用于检测生物膜产生。
约 16.4%的铜绿假单胞菌分离株和 22%的不动杆菌属菌株为金属β-内酰胺酶产生菌。85 株铜绿假单胞菌中有 23 株（27.05%）根据管附着试验为生物膜产生菌，而仅 13 株（15.29%）根据刚果红琼脂法为生物膜产生菌。同样，50 株不动杆菌属中有 7 株（14%）根据管附着试验为生物膜产生菌，而仅 5 株（10%）根据刚果红琼脂法为生物膜产生菌。铜绿假单胞菌和不动杆菌属对黏菌素的敏感性最高。
在本研究中，铜绿假单胞菌和不动杆菌属产生生物膜和金属β-内酰胺酶。然而，生物膜产生与金属β-内酰胺酶产生之间没有建立统计学上的显著相关性。
