Lin Xiang, Xia Wei, Ji Wei, Xie Fang-Fei, He Pei, Zhu Xiao-Wei, Zhang Yong-Hong, Deng Fei-Yan, Lei Shu-Feng
Center for Genetic Epidemiology & Genomics, School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, PR China.
Jiangsu Key Laboratory of Preventive & Translational Medicine for Geriatric Diseases, School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, PR China.
Epigenomics. 2017 Oct;9(10):1287-1298. doi: 10.2217/epi-2017-0042. Epub 2017 Sep 7.
To detect SNP-miRNA-mRNA interaction networks and to elucidate miRNA-mediated regulation effects on mRNA expression.
MATERIALS & METHODS: In human peripheral blood mononuclear cells of 43 females, SNP-miRNA-mRNA interaction networks were established through an integrative analysis. Then causal inference test was followed to detect miRNA-mediated effects on mRNA expressions.
About 167 trios corresponding to 56 SNPs, 20 miRNAs and 47 target-mRNAs have the SNP-miRNA-mRNA interactions, but only 22 trios have miRNA-mediated effects between SNP and mRNA. For the three miRNAs (hsa-miR-222-3p, hsa-miR-181b-5p and hsa-miR-106b-5p), each mediates at least four correlations between SNP and mRNA. The mRNAs in interaction play an important role in energy metabolism, cellular and tissue homeostasis.
This study represents the first effort of constructing an integrative interaction network of SNP-miRNA-mRNA and miRNA-mediated regulatory effects that provide helpful clues for future investigations of peripheral blood mononuclear cell-related physiological process and immunological diseases.
检测单核苷酸多态性（SNP）-微小RNA（miRNA）-信使核糖核酸（mRNA）相互作用网络，并阐明miRNA对mRNA表达的介导调控作用。
在43名女性的人外周血单个核细胞中，通过综合分析建立SNP-miRNA-mRNA相互作用网络。然后进行因果推断测试，以检测miRNA对mRNA表达的介导作用。
约167个三联体对应56个SNP、20个miRNA和47个靶mRNA存在SNP-miRNA-mRNA相互作用，但只有22个三联体在SNP和mRNA之间存在miRNA介导的作用。对于三种miRNA（hsa-miR-222-3p、hsa-miR-181b-5p和hsa-miR-106b-5p），每种miRNA至少介导SNP和mRNA之间的四种相关性。相互作用中的mRNA在能量代谢、细胞和组织稳态中起重要作用。
本研究首次构建了SNP-miRNA-mRNA综合相互作用网络以及miRNA介导的调控作用，为未来外周血单个核细胞相关生理过程和免疫疾病的研究提供了有益线索。
