Park Hyun Jung, Lee Sung Won, Kwon Dong-Joo, Heo Seong-Il, Park Se-Ho, Kim Sun Young, Hong Seokmann
Department of Integrative Bioscience and Biotechnology, Institute of Anticancer Medicine Development, Sejong University, Seoul, 143-747, South Korea.
School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, South Korea.
BMC Complement Altern Med. 2017 Sep 6;17(1):448. doi: 10.1186/s12906-017-1952-4.
Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders that are mediated by pathogenic Th1 and Th17 cells. Previous studies have demonstrated that taheebo water extract (TWE) derived from Tabebuia avellanedae Lorentz ex Griseb., as folk remedy, has been used to treat various inflammatory diseases. Although TWE has been previously shown to display anti-inflammatory activities, the in vivo effects of TWE on mucosal immune responses remain unclear.
We examined the anti-inflammatory effects of TWE on innate immune cells such as dendritic cells (DCs) and macrophages and also on the differentiation of T helper cells. Lastly, adopting a method for dextran sulfate sodium (DSS)-induced colitis, we investigated whether the oral administration of TWE can modulate mucosal inflammatory responses.
We found that TWE could activate DCs to produce immunosuppressive IL10 and polarize macrophages toward an anti-inflammatory phenotype in the mesenteric lymph node (MLN). Such alterations in DCs and macrophages resulted in a significant increase in anti-inflammatory Th2 and Foxp3 Treg cells and a dramatic decrease in pro-inflammatory Th1 and Th17 cells in the MLN. Upon induction of colitis with DSS treatment, TWE significantly reduced the clinical symptoms, including body weight loss and colonic tissue inflammation, by up-regulating type II T helper immune responses.
Taken together, these data suggest that TWE is an excellent natural product with therapeutic effects to help improve inflammatory disorders such as colitis.
炎症性肠病（IBD）是由致病性Th1和Th17细胞介导的慢性炎症性疾病。先前的研究表明，从椭圆叶紫葳（Tabebuia avellanedae Lorentz ex Griseb.）中提取的太赫兹水提取物（TWE）作为民间疗法，已被用于治疗各种炎症性疾病。尽管TWE先前已显示出抗炎活性，但其对黏膜免疫反应的体内作用仍不清楚。
我们研究了TWE对天然免疫细胞（如树突状细胞（DC）和巨噬细胞）以及对辅助性T细胞分化的抗炎作用。最后，采用葡聚糖硫酸钠（DSS）诱导结肠炎的方法，我们研究了口服TWE是否能调节黏膜炎症反应。
我们发现TWE可以激活DC产生免疫抑制性IL10，并使肠系膜淋巴结（MLN）中的巨噬细胞向抗炎表型极化。DC和巨噬细胞的这种改变导致MLN中抗炎性Th2和Foxp3调节性T细胞显著增加，而促炎性Th1和Th17细胞显著减少。在用DSS诱导结肠炎后，TWE通过上调II型辅助性T细胞免疫反应，显著减轻了包括体重减轻和结肠组织炎症在内的临床症状。
综上所述，这些数据表明TWE是一种具有治疗作用的优秀天然产物，有助于改善结肠炎等炎症性疾病。
