Liang Zhibin, Zhang Yang, Song Jie, Zhang Hui, Zhang Suzhen, Li Yue, Tan Juan, Qiao Wentao
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
College of Life Sciences, Nankai University, 94 Weijin Rd, Tianjin, 300071, China.
Virol J. 2017 Sep 6;14(1):173. doi: 10.1186/s12985-017-0835-0.
Human BST2 (hBST2, also called Tetherin) is a host restriction factor that blocks the release of various enveloped viruses. BST2s from different mammals also possess antiviral activity. Bovine BST2s (bBST2s), bBST2A1 and bBST2A2, reduce production of cell-free bovine leukemia virus (BLV) and vesicular stomatitis virus (VSV). However, the effect of bBST2 on other retroviruses remains unstudied.
Here, we studied the antiviral activity of wildtype and mutant bBST2A1 proteins on retroviruses including human immunodeficiency virus type 1 (HIV-1), prototypic foamy virus (PFV), bovine foamy virus (BFV) and bovine immunodeficiency virus (BIV). The results showed that wildtype bBST2A1 suppressed the release of HIV-1, PFV and BFV. We also generated bBST2A1 mutants, and found that GPI anchor and dimerization, but not glycosylation, are essential for antiviral activity of bBST2A1. Moreover, unlike hBST2, bBST2A1 displayed no inhibitory effect on cell-to-cell transmission of PFV, BFV and BIV.
Our data suggested that bBST2A1 inhibited retrovirus release, however, had no effect on cell-to-cell transmission of retroviruses.
人类BST2（hBST2，也称为栓系蛋白）是一种宿主限制因子，可阻止各种包膜病毒的释放。来自不同哺乳动物的BST2也具有抗病毒活性。牛BST2（bBST2），即bBST2A1和bBST2A2，可减少无细胞牛白血病病毒（BLV）和水疱性口炎病毒（VSV）的产生。然而，bBST2对其他逆转录病毒的作用仍未得到研究。
在此，我们研究了野生型和突变型bBST2A1蛋白对包括1型人类免疫缺陷病毒（HIV-1）、原型泡沫病毒（PFV）、牛泡沫病毒（BFV）和牛免疫缺陷病毒（BIV）在内的逆转录病毒的抗病毒活性。结果表明，野生型bBST2A1可抑制HIV-1、PFV和BFV的释放。我们还构建了bBST2A1突变体，发现糖基磷脂酰肌醇（GPI）锚定和二聚化而非糖基化对bBST2A1的抗病毒活性至关重要。此外，与hBST2不同，bBST2A1对PFV、BFV和BIV的细胞间传播没有抑制作用。
我们的数据表明，bBST2A1可抑制逆转录病毒释放，但对逆转录病毒的细胞间传播没有影响。
