Fang Zhengyu, He Dongyi, Yu Bo, Liu Feng, Zuo Jianping, Li Yuxia, Lin Qi, Zhou Xiaodong, Wang Qingwen
Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen 518036, China.
Department of Rheumatism and Immunology, Peking University Shenzhen Hospital, Shenzhen 518036, China.
Genes (Basel). 2017 Sep 6;8(9):221. doi: 10.3390/genes8090221.
Celastrol, a natural triterpene, exhibits potential anti-inflammatory activity in a variety of inflammatory diseases. The present study aimed to investigate its biological effect on activated fibroblast-like synoviocytes (FLSs) from patients with rheumatoid arthritis (RA). The primary FLSs of the synovial tissues were obtained from synovial biopsies of patients with RA. The normal human FLS line (HFLS) was used as a control. After the RA-FLSs and HFLSs were treated with or without celastrol, various approaches, including the WST-1 assay, transwell assay, real-time PCR and ELISA analysis, were performed to estimate proliferation, invasion and expression of pro-inflammatory cytokines of the RA-FLSs. Microarray analysis was performed to screen for differentially expressed genes in RA-FLSs before and after celastrol treatment. The results showed that treatment of celastrol attenuated both the proliferation and invasion of the RA-FLSs. The expression of several chemokine genes, including , , , and , was significantly changed after celastrol treatment. The genes involved in the NF-κB signaling pathway appeared to be regulated by celastrol.
雷公藤红素是一种天然三萜类化合物，在多种炎症性疾病中表现出潜在的抗炎活性。本研究旨在探讨其对类风湿关节炎（RA）患者活化的成纤维样滑膜细胞（FLS）的生物学作用。滑膜组织的原代FLS取自RA患者的滑膜活检样本。正常人FLS系（HFLS）用作对照。在用或不用雷公藤红素处理RA-FLS和HFLS后，采用多种方法，包括WST-1检测、Transwell检测、实时PCR和ELISA分析，来评估RA-FLS的增殖、侵袭及促炎细胞因子的表达。进行基因芯片分析以筛选雷公藤红素处理前后RA-FLS中差异表达的基因。结果表明，雷公藤红素处理可减弱RA-FLS的增殖和侵袭。雷公藤红素处理后，包括 、 、 、 和 在内的几种趋化因子基因的表达发生了显著变化。参与NF-κB信号通路的基因似乎受雷公藤红素调控。
