Department of Biochemistry and Molecular Biophysics, Columbia University 650 West 168th Street, New York, NY, 10032, USA.
Sci Rep. 2017 Sep 6;7(1):10669. doi: 10.1038/s41598-017-10993-9.
Tetrameric ion channels have either swapped or non-swapped arrangements of the S1-S4 and pore domains. Here we show that mutations in the transmembrane domain of TRPV6 can result in conversion from a domain-swapped to non-swapped fold. These results reveal structural determinants of domain swapping and raise the possibility that a single ion channel subtype can fold into either arrangement in vivo, affecting its function in normal or disease states.
四聚体离子通道的 S1-S4 和孔道结构域存在交换或未交换的排列。在这里，我们表明 TRPV6 跨膜结构域的突变可导致从交换折叠到非交换折叠的转变。这些结果揭示了结构域交换的决定因素，并提出了这样一种可能性，即单一的离子通道亚型可以在体内折叠成任意一种构象，从而影响其在正常或疾病状态下的功能。
