Jin Ji-Hui, Cheng Jin-Long, He Zi-Rong, Ren Ying-Chao, Yu Xiao-Hui, Song Yang, Yang Hui-Ming, Yang Yan-Ling, Liu Tong, Zhang Guo-Zhong
Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural UniversityBeijing, China.
Diagnostic and Research Center of Livestock and Poultry Epidemic Diseases, China Agricultural UniversityBeijing, China.
Front Microbiol. 2017 Aug 23;8:1607. doi: 10.3389/fmicb.2017.01607. eCollection 2017.
To investigate the exact effects of different origins of Newcastle disease virus (NDV) hemagglutinin-neuraminidase (HN) protein to the biological characteristics of the virus, we systematically studied the correlation between the HN protein and NDV virulence by exchanging the HN of velogenic or lentogenic NDV strains with the HN from other strains of different virulence. The results revealed that the rSG10 or rLaSota derivatives bearing the HN gene of other viruses exhibited decreased or increased hemadsorption (HAd), neuraminidase and fusion promotion activities. and tests further showed that changes in replication level, tissue tropism and virulence of the chimeric viruses were also consistent with these biological activities. These findings demonstrated that the balance among three biological activities caused variation in replication and pathogenicity of the virus, which was closely related to the origin of the HN protein. Our study highlights the importance of the HN glycoprotein in modulating the virulence of NDV and contributes to a more complete understanding of the virulence of NDV.
为研究不同来源的新城疫病毒（NDV）血凝素神经氨酸酶（HN）蛋白对病毒生物学特性的具体影响，我们通过将强毒株或弱毒株的HN与其他不同毒力毒株的HN进行交换，系统研究了HN蛋白与NDV毒力之间的相关性。结果显示，携带其他病毒HN基因的rSG10或rLaSota衍生物表现出血凝吸附（HAd）、神经氨酸酶及融合促进活性降低或升高。 试验进一步表明，嵌合病毒在复制水平、组织嗜性和毒力方面的变化也与这些生物学活性一致。这些发现表明，三种生物学活性之间的平衡导致了病毒复制和致病性的变化，这与HN蛋白的来源密切相关。我们的研究突出了HN糖蛋白在调节NDV毒力中的重要性，并有助于更全面地了解NDV的毒力。
