Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110020, India.
Council of Scientific and Industrial Research (CSIR)-Central Scientific Instruments Organisation, Chandigarh, 160030, India.
Appl Microbiol Biotechnol. 2017 Oct;101(20):7635-7652. doi: 10.1007/s00253-017-8473-4. Epub 2017 Sep 6.
Probiotic industries strive for new, efficient and promising probiotic strains that impart a positive impact on consumer health. Challenges are persisting in isolation, screening, and selection of the new indigenous probiotic strains. In the present research, we explored the probiotic potential of 17 lactic acid bacteria isolated from Yak milk in a series of in vitro tests. We also demonstrated their health benefits, i.e., cholesterol degradation, lactose digestion, antimicrobial activity, antioxidant, and anticancer activities. Principal component analysis revealed that more than 50% of the strains fulfilled the examined criteria, e.g., survival in acidic pH, bile concentrations, and adherent property. Approximately all the strains produced antimicrobial substances against the maximum number of tested strains including clinical strains. Most strains degraded cholesterol in comparison to the reference probiotic strain whereas strain Yc showed 1.5 times higher the degradation efficiency of the control strain. Lan4 strain exhibited remarkable anticancer activity and induced the maximum apoptosis (87%) in the Hela cells and was non-toxic to the non-cancerous HEK293 cells. Around ten strains showed positive lactose digestion. Overall, this can be concluded that selected lactic acid bacteria revealed excellent probiotic properties along with desirable health benefits. These strains need to be further investigated in details for their application in the development of novel probiotic preparations for the improvement of public health.
益生菌产业致力于寻找新的、高效的、有前景的益生菌菌株，以期对消费者的健康产生积极影响。目前，在益生菌新菌株的分离、筛选和选择方面仍存在诸多挑战。在本研究中，我们通过一系列体外试验，对从牦牛乳中分离得到的 17 株乳酸菌的益生菌潜力进行了探索。此外，还对其健康益处，如胆固醇降解、乳糖消化、抗菌、抗氧化和抗癌活性进行了研究。主成分分析表明，超过 50%的菌株满足了所检查的标准，例如在酸性 pH 值、胆汁浓度和黏附性方面的生存能力。几乎所有的菌株都产生了抗菌物质，对抗生素耐药的临床菌株也具有抑制作用。与参考益生菌菌株相比，大多数菌株都能降解胆固醇，而菌株 Yc 对胆固醇的降解效率比对照菌株高出 1.5 倍。Lan4 菌株表现出显著的抗癌活性，在 Hela 细胞中诱导了最高的凋亡（87%），且对非癌细胞 HEK293 无毒性。大约有 10 株菌株表现出阳性的乳糖消化能力。总的来说，本研究可以得出结论，所选的乳酸菌具有良好的益生菌特性和理想的健康益处。这些菌株需要进一步深入研究，以便将其应用于新型益生菌制剂的开发，以改善公众健康。
