Vasundaradevi R, Sarvajith M, Somashekaraiah Rakesh, Gunduraj Adithi, Sreenivasa M Y
Applied Mycology Laboratory, Department of Studies in Microbiology, University of Mysore, Mysuru, India.
Front Microbiol. 2024 Feb 9;15:1322758. doi: 10.3389/fmicb.2024.1322758. eCollection 2024.
, a notorious phytopathogenic fungus, has been documented to infect several plant species, leading to the loss of agricultural commodities and resulting in significant economic losses. Lactic acid bacteria (LAB) hold immense promise as biocontrol candidates. However, the potential of LABs derived from fruits remains largely unexplored. In this study, several LABs were isolated from tropical fruit and assessed for their probiotic and antifungal properties. A total of fifty-five LABs were successfully isolated from seven distinct fruits. Among these, seven isolates showed inhibition to growth of . Two strains, isolated from fruits: , and exhibited promising antifungal properties against . Molecular identification confirmed their identities as MYSVB1 and MYSVA7, respectively. Both strains showed adaptability to a wide temperature range (10-45°C), and salt concentrations (up to 7%), with optimal growth around 37 °C and high survival rates under simulated gastrointestinal conditions. Among these two strains, MYSVB1 demonstrated significant inhibition ( < 0.01) of the growth of . The inhibitory effects of cell-free supernatant (CFS) were strong, with 5% crude CFS sufficient to reduce fungal growth by >70% and complete inhibition by 10% CFS. Moreover, the CFS was inhibitory for both mycelial growth and conidial germination. CFS retained its activity even after long cold storage. The chromatographic analysis identified organic acids in CFS, with succinic acid as the predominant constituent, with lactic acid, and malic acid in descending order. LAB strains isolated from tropical fruits showed promising probiotic and antifungal properties, making them potential candidates for various applications in food and agriculture.
一种臭名昭著的植物病原真菌已被证明可感染多种植物物种，导致农产品损失并造成重大经济损失。乳酸菌（LAB）作为生物防治候选菌具有巨大潜力。然而，源自水果的乳酸菌的潜力在很大程度上仍未得到探索。在本研究中，从热带水果中分离出几种乳酸菌，并评估了它们的益生菌和抗真菌特性。总共从七种不同的水果中成功分离出五十五株乳酸菌。其中，七株分离物对[真菌名称未给出]的生长表现出抑制作用。从水果中分离出的两株菌株：[菌株名称未给出]和[菌株名称未给出]对[真菌名称未给出]表现出有前景的抗真菌特性。分子鉴定分别证实它们的身份为MYSVB1和MYSVA7。两株菌株均显示出对广泛温度范围（10 - 45°C）和盐浓度（高达7%）的适应性，在37°C左右生长最佳，在模拟胃肠道条件下存活率高。在这两株菌株中，MYSVB1对[真菌名称未给出]的生长表现出显著抑制（P < 0.01）。无细胞上清液（CFS）的抑制作用很强，5%的粗CFS足以使真菌生长减少>70%，10%的CFS可完全抑制。此外，CFS对菌丝体生长和分生孢子萌发均有抑制作用。即使经过长时间冷藏，CFS仍保持其活性。色谱分析鉴定出CFS中的有机酸，以琥珀酸为主要成分，其次是乳酸和苹果酸。从热带水果中分离出的乳酸菌菌株显示出有前景的益生菌和抗真菌特性，使其成为食品和农业中各种应用的潜在候选菌。
