Han Yan-Zhong, Zhou Yong-Feng, Sang Xiu-Xiu, Liu Hui-Min, Cui He-Rong, Meng Ya-Kun, Li Guang-Quan, He Lan-Zhi, Yin Ping, Wang Jia-Bo, Bai Zhao-Fang, Xiao Xiao-He
Chengde Medical college, Chengde 067000, China.
China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing 100039, China.
Zhongguo Zhong Yao Za Zhi. 2016 Apr;41(7):1302-1307. doi: 10.4268/cjcmm20160723.
To investigate the protective effects of oxymatrine (OMT) against H2O2-induced damage in L02 cells and research the mechanism,L02 cells were used as the research object. The oxidative stress model of L02 was established by hydrogen peroxide (H2O2). CCK-8 was used to detect the cell activation of L02 cells treated by different OMT. FCM (flow cytometry) assay was used to evaluate the cell proliferation of L02 cells treated by OMT. The apoptosis of L02 cells was detected using Annexin-V/7-AAD apoptosis detection kit. The level of ROS was detected by DCFH-DA fluorescence probe. The GSH-PX and SOD were detected by micro plate and colorimetric method. Results showed that when the concentration of OMT is between 6.25 and 100 mg•L⁻¹, it could promote the production of NADPH and strengthen the activity of GSH-PX and SOD to get rid of the ROS to protect the L02 cell from the apoptosis of L02 cell induced by H2O2.
为研究氧化苦参碱（OMT）对H2O2诱导的L02细胞损伤的保护作用并探究其机制，以L02细胞作为研究对象。用过氧化氢（H2O2）建立L02细胞的氧化应激模型。采用CCK-8检测不同浓度OMT处理的L02细胞的细胞活性。采用流式细胞术（FCM）检测OMT处理的L02细胞的增殖情况。使用Annexin-V/7-AAD凋亡检测试剂盒检测L02细胞的凋亡情况。用DCFH-DA荧光探针检测活性氧（ROS）水平。采用微孔板和比色法检测谷胱甘肽过氧化物酶（GSH-PX）和超氧化物歧化酶（SOD）。结果表明，当OMT浓度在6.25至100 mg•L⁻¹之间时，可促进烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的产生，增强GSH-PX和SOD的活性以清除ROS，从而保护L02细胞免受H2O2诱导的L02细胞凋亡。
