Wang Qian, Wang Xiaohong, An Jingjing, Wang Chen, Wang Feng
Department of Nutrition, Second Sanatorium of Qingdao Navy, Qingdao 266001, China.
Wei Sheng Yan Jiu. 2018 Mar;47(2):281-306.
To explore lycopene 's protective effect on H_2O_2 induced oxidative damage of L02 cells and its mechanism.
L02 cells were cultured by H_2O_2 to build the model of cellular oxidative damage. Different dose of lycopene was used to pretreat the cells, and cell survival rate was detected to verify the appropriate concentration. Then cellular ROS level, activity of cellular SOD and GSH-Px, cellular MDA content, and activity of ALT, AST and LDH in the culture medium were detected to observe lycopene 's effect on cellular oxidant damage. Finally, to observe lycopene 's activating effect on nuclear-translocation of Nrf2, L02 cells' nuclear protein was extracted to detect Nrf2 protein content, and also, mRNA expression level of Nrf2 target genes HO-1 and NQO1 was assayed to verify this mechanism.
Pretreatment of 10 μmol/Llycopene raised cellular viability of L02 cells on H_2O_2 culturing condition, reduced cellular ROS, enhanced enzymatic activity of cellular SOD and GSH-Px, reduced cellular MDA content, and depressed the activity of ALT, AST and LDH in culture medium. Lycopene also increased nuclear Nrf2 protein content and enhanced the expression of its target genes HO-1 and NQO1.
Lycopene could protect L02 cells from H_2O_2 induced oxidative damage, probably by promoting nuclear-translocation of Nrf2 and activating expression of its target antioxidant genes.
探讨番茄红素对H₂O₂诱导的L02细胞氧化损伤的保护作用及其机制。
用H₂O₂培养L02细胞建立细胞氧化损伤模型。用不同剂量的番茄红素预处理细胞，检测细胞存活率以确定合适的浓度。然后检测细胞内活性氧水平、细胞超氧化物歧化酶和谷胱甘肽过氧化物酶活性、细胞丙二醛含量以及培养基中谷丙转氨酶、谷草转氨酶和乳酸脱氢酶活性，以观察番茄红素对细胞氧化损伤的影响。最后，为观察番茄红素对Nrf2核转位的激活作用，提取L02细胞核蛋白检测Nrf2蛋白含量，并检测Nrf2靶基因HO-1和NQO1的mRNA表达水平以验证该机制。
10 μmol/L番茄红素预处理可提高H₂O₂培养条件下L02细胞的活力，降低细胞内活性氧，增强细胞超氧化物歧化酶和谷胱甘肽过氧化物酶的酶活性，降低细胞丙二醛含量，并降低培养基中谷丙转氨酶、谷草转氨酶和乳酸脱氢酶的活性。番茄红素还增加了细胞核Nrf2蛋白含量并增强了其靶基因HO-1和NQO1的表达。
番茄红素可保护L02细胞免受H₂O₂诱导的氧化损伤，可能是通过促进Nrf2核转位并激活其靶抗氧化基因的表达来实现的。
