Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan, and Oilseeds Research Program, National Agricultural Research Centre, Islamabad, Pakistan.
Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan.
Curr Issues Mol Biol. 2018;26:47-54. doi: 10.21775/cimb.026.047. Epub 2017 Sep 7.
Recently the engineered nucleases have revolutionized genome editing to perturb gene expression at specific sites in complex eukaryotic genomes. Three important classes of these genome editing tools are Moreover, the more recent type II Clustered Regularly Inter-spaced Short Palindromic Repeats/Crispr associated protein (CRISPR/Cas9) system has become the most favorite plant genome editing tool for its precision and RNA based specificity unlike its counterparts which rely on protein based specificity. Plasmid-mediated co-delivery of multiple sgRNAs and Cas9 to the Plant cell can simultaneously alter more than one target loci which enable multiplex genome editing. In this review, we discuss recent advancements in the CRISPR/ Cas9 technology mechanism, theory and its applications in plants and agriculture. We also suggest that the CRISPR/ Cas9 as an effective genome editing tool, has vast potential for crop improvement and studying gene regulation mechanism and chromatin remodeling.
最近，经过工程改造的核酸酶在复杂真核生物基因组中特定位置上的基因表达的基因编辑方面带来了革命性的变化。这些基因编辑工具的三个重要类别是：此外，最近出现的 II 型 Clustered Regularly Inter-spaced Short Palindromic Repeats/Crispr 相关蛋白（CRISPR/Cas9）系统已成为最受欢迎的植物基因组编辑工具，因为其精度和基于 RNA 的特异性，与依赖于基于蛋白的特异性的其他系统不同。通过质粒介导将多个 sgRNA 和 Cas9 共递送到植物细胞中，可以同时改变一个以上的靶标基因座，从而实现多重基因组编辑。在本文中，我们讨论了 CRISPR/Cas9 技术的机制、理论及其在植物和农业中的应用方面的最新进展。我们还建议将 CRISPR/Cas9 作为一种有效的基因组编辑工具，具有巨大的潜力用于作物改良以及研究基因调控机制和染色质重塑。
