Paul Joseph W, Qi Yiping
Department of Biology, Thomas Harriot College of Arts and Sciences, East Carolina University, Greenville, NC, 27858, USA.
Plant Cell Rep. 2016 Jul;35(7):1417-27. doi: 10.1007/s00299-016-1985-z. Epub 2016 Apr 25.
The increasing burden of the world population on agriculture requires the development of more robust crops. Dissecting the basic biology that underlies plant development and stress responses will inform the design of better crops. One powerful tool for studying plants at the molecular level is the RNA-programmed genome editing system composed of a clustered regularly interspaced short palindromic repeats (CRISPR)-encoded guide RNA and the nuclease Cas9. Here, some of the recent advances in CRISPR/Cas9 technology that have profound implications for improving the study of plant biology are described. These tools are also paving the way towards new horizons for biotechnologies and crop development.
世界人口对农业造成的负担日益加重，这就需要培育出更强健的作物。剖析植物发育和应激反应背后的基础生物学原理，将为培育更优良的作物提供依据。在分子水平上研究植物的一种强大工具是由成簇规律间隔短回文重复序列（CRISPR）编码的引导RNA和核酸酶Cas9组成的RNA编程基因组编辑系统。本文介绍了CRISPR/Cas9技术最近取得的一些进展，这些进展对改进植物生物学研究具有深远意义。这些工具也为生物技术和作物培育的新发展铺平了道路。
