Murdoch T B, McGhee-Wilson D, Shapiro A M J, Lakey J R T
Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
Cell Transplant. 2004 Sep;13(6):605-618. doi: 10.3727/000000004783983602.
The ability to maintain isolated human islet preparations in tissue culture has recently been adopted by most islet transplant centers, and improves the safety as well as the practicality of islet transplantation. Maintaining islet viability and recovery, however, remains challenging in a clinical setting, due to stringent conditions required for culture. Islet culture is further complicated by the fact that islets do not form a monolayer. This review aims to clarify media, supplementation, and conditions that have been shown to be relevant to human islets, as well as to offer avenues of future research. Factors examined that may influence islet survival include base medium, glucose concentration, vitamin, inorganic ion, lipid, hormone, growth factor, amino acid, and binding protein composition and concentration, as well as culture temperature and seeding density. In addition, this article reviews novel techniques, such as coculture and matrices, that have been employed in an attempt to improve islet survival and functional viability.
目前，大多数胰岛移植中心已采用在组织培养中维持分离的人胰岛制剂的方法，这提高了胰岛移植的安全性和实用性。然而，由于培养所需的严格条件，在临床环境中维持胰岛活力和恢复仍然具有挑战性。胰岛不形成单层这一事实使胰岛培养更加复杂。本综述旨在阐明已被证明与人类胰岛相关的培养基、补充剂和条件，并提供未来研究的途径。研究的可能影响胰岛存活的因素包括基础培养基、葡萄糖浓度、维生素、无机离子、脂质、激素、生长因子、氨基酸和结合蛋白的组成与浓度，以及培养温度和接种密度。此外，本文还综述了为提高胰岛存活和功能活力而采用的新技术，如共培养和基质。
