在旋角羚（Addax nasomaculatus，羚羊亚科）初乳中鉴定包括寡糖核苷酸在内的唾液酸寡糖。
Identification of sialyl oligosaccharides including an oligosaccharide nucleotide in colostrum of an addax (Addax nasomaculatus) (Subfamily Antelopinae).
作者信息
Ganzorig Khuukhenbaatar, Asakawa Takuya, Sasaki Masashi, Saito Tadao, Suzuki Isao, Fukuda Kenji, Urashima Tadasu
机构信息
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Obihiro, Hokkaido, Japan.
Himeji Central Park, Toyotomicho Konandai, Himeji, Hyogo, Japan.
出版信息
Anim Sci J. 2018 Jan;89(1):167-175. doi: 10.1111/asj.12899. Epub 2017 Sep 7.
Mammalian milk/colostrum usually contains milk oligosaccharides along with the predominant lactose. Although milk oligosaccharides of a variety of Bovidae species including cow, sheep and goat have been characterized, those of the addax, an Antelopinae species of the Bovidae, have not as yet been clarified. In this study, several sialyl oligosaccharides were purified from a sample of addax colostrum and characterized as follows: Neu5Ac(α2-8)Neu5Ac(α2-3)Gal(β1-4)Glc, Neu5Gc(α2-8)Neu5Gc(α2-3)Gal(β1-4)Glc, Neu5Ac(α2-3)Gal(β1-4)Glc, Neu5Ac(α2-6)Gal(β1-4)GlcNAc, Neu5Gc(α2-3)Gal(β1-4)Glc, Neu5Gc(α2-6)Gal(β1-4)Glc, Neu5Gc(α2-6)Gal(β1-4)GlcNAc. In addition, an oligosaccharide nucleotide Neu5Gc(α2-6)Gal(β1-4)GlcNAcα1-UDP was characterized. Molecular species of a variety of sialyl oligosaccharides found in milk and colostrum of these Bovidae were compared.
哺乳动物的乳汁/初乳通常除了含有占主导地位的乳糖外，还含有乳寡糖。尽管包括牛、绵羊和山羊在内的多种牛科动物的乳寡糖已被鉴定，但旋角羚（牛科羚羊亚科的一种）的乳寡糖尚未得到阐明。在本研究中，从旋角羚初乳样品中纯化出了几种唾液酸化寡糖，并对其进行了如下鉴定：Neu5Ac(α2-8)Neu5Ac(α2-3)Gal(β1-4)Glc、Neu5Gc(α2-8)Neu5Gc(α2-3)Gal(β1-4)Glc、Neu5Ac(α2-3)Gal(β1-4)Glc、Neu5Ac(α2-6)Gal(β1-4)GlcNAc、Neu5Gc(α2-3)Gal(β1-4)Glc、Neu5Gc(α2-6)Gal(β1-4)Glc、Neu5Gc(α2-6)Gal(β1-4)GlcNAc。此外，还鉴定了一种寡糖核苷酸Neu5Gc(α2-6)Gal(β1-4)GlcNAcα1-UDP。对这些牛科动物的乳汁和初乳中发现的多种唾液酸化寡糖的分子种类进行了比较。
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