一种用于检测人类粪便样本中溶组织内阿米巴抗原的双抗体夹心酶联免疫吸附测定法。

A double-antibody sandwich ELISA for the detection of Entamoeba histolytica antigen in stool samples of humans.

作者信息

Baumann D, Gottstein B

出版信息

Trop Med Parasitol. 1987 Jun;38(2):81-5.

Abstract

A double-antibody sandwich ELISA was developed to detect detergent-solubilized antigens of Entamoeba histolytica in stool samples of humans. The test system was evaluated for its methodical and diagnostic sensitivity and specificity. In recovery experiments the lower limit of detection was 400 ng E. histolytica (HK9) protein/ml stool, corresponding to approximately 2000 amoebic trophozoites/ml stool. Samples of 97 patients with suspected intestinal amoebiasis were examined. Specific antigens were detected by ELISA (= positive reaction) in 14 (93%) out of 15 stool samples containing trophozoites of E. histolytica. In contrast, 68 (93%) of 73 samples with other protozoa, including Entamoeba coli, E. hartmanni, Endolimax nana, Iodamoeba buetschlii and Giardia lamblia, did not react in the test system (= negative reaction). The test was shown to detect only trophozoites of E. histolytica and not the cyst stage. This fact could facilitate the differentiation between cyst carriers and persons excreting trophozoites. The results of this preliminary study justify a further large scale evaluation of the test system.

摘要

开发了一种双抗体夹心酶联免疫吸附测定法（ELISA），用于检测人类粪便样本中经去污剂溶解的溶组织内阿米巴抗原。对该检测系统的方法学、诊断敏感性和特异性进行了评估。在回收率实验中，检测下限为每毫升粪便400纳克溶组织内阿米巴（HK9）蛋白，相当于每毫升粪便约2000个阿米巴滋养体。对97例疑似肠道阿米巴病患者的样本进行了检测。在15份含有溶组织内阿米巴滋养体的粪便样本中，有14份（93%）通过ELISA检测到特异性抗原（=阳性反应）。相比之下，在73份含有其他原生动物（包括结肠内阿米巴、哈氏内阿米巴、微小内蜒阿米巴、布氏嗜碘阿米巴和蓝氏贾第鞭毛虫）的样本中，有68份（93%）在检测系统中无反应（=阴性反应）。该检测方法仅能检测到溶组织内阿米巴的滋养体，而不能检测到包囊阶段。这一事实有助于区分包囊携带者和排出滋养体的人。这项初步研究的结果证明对该检测系统进行进一步大规模评估是合理的。